PsaA and PsaB are central proteins in photosystem 1,and play important roles in plant photosynthesis and stress regulation.Tobacco(Nicotiana tabacum L.)is a model crop for plant functional genomics research,while there are few studies on the functions of PsaA and PsaB genes.In order to understand the response of tobacco PsaA/PsaB genes to stress,the PsaA/PsaB gene family in tobacco genome were identified,and their physicochemical properties,subcellular localization,phylogenetic evolution,motifs and promoter regulatory elements were analyzed.The results showed that there are 27 PsaA/PsaB genes in tobacco.Compared with Arabidopsis thaliana,the tobacco PsaA/PsaB gene family undergone significant expansion and can be classified into three subfamilies based on their phylogenetic and structural features.The promoters of tobacco PsaA/PsaB genes contain numerous cis-regulatory elements responsive to light,low temperature,drought and plant hormones.RT-qPCR analysis revealed that most tobacco PsaA/PsaB genes were up-regulated under low temperature stress(8 up-regulated genes)and down-regulated under polyethylene glycol and jasmonic acid methyl ester stresses(8 and 7 down-regulated genes respectively).After potato virus Y(PVY)and tobacco mosaic virus(TMV)infection,the expression of most tobacco PsaA/PsaB genes were down-regulated.The down-regulated genes in the lower mesophyll and vein tissues were 19 and 17 after PVY infection,and 9 genes were down-regulated after TMV infection.In conclusion,tobacco PsaA/PsaB genes are involved in the response process of tobacco to various abiotic stresses,and the study results provide a reference for further exploring the role of PsaA/PsaB genes in regulating plant response to stress.
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