Rapid and accurate detection for Staphylococcus aureus in drugs based on real-time fluorescent quantitative PCR
In order to achieve accurate and rapid detection for Staphylococcus aureus in drugs,through adding internal amplification control(IAC)and optimizing PCR reaction system,an internal reference based fluorescence quantitative PCR rapid detection method of Staphylococcus aureus that can monitor the process in real time was established,and its specificity,detection limit,reproducibility and feasibility were verified.The verification results showed that the specificity of the established method was good with only Staphylococcus aureus showed typical amplification curve.The detection limit of Staphylococcus aureus genomic DNA was 0.23 pg/μL.There was a good linear relationship between C,values and the copy numbers of template(R2=0.99).The relative standard deviations of reproducibility experiments were all less than 3.0％.Staphylococcus aureus can be detected 10 h after the bacteria increase in artificially contaminated drugs.The method can not only shorten the detection time of Staphylococcus aureus in drugs,but can also monitor the PCR reaction process,effectively prevent the occurrence of"false negative"result,thus can be used as a supplementary method for staphylococcus aureus detection.
万方数据
维普
