Experimental preparation,a basic step of experiment procedure,is the guarantee for smooth operation of postgraduate pro-jects.Although experimental teaching is the foundation of medical talents cultivation,experimental preparation tends to be neglected in the traditional teaching of experimental courses for undergraduate and postgraduate students.In view of the above-mentioned problem,the authors explored the experimental preparation in the flipped experimental teaching of postgraduate professional courses.The post-graduates'knowledge reserve and their research need for research projects make the teaching reform feasible and necessary.With the new teaching mode,the postgraduates can not only master key experimental techniques,but also develop experimental preparation abil-ities in the aspects of literature retrieval,experimental protocols,reagent preparation,and pilot study.The teaching reform helps to im-prove the comprehensive abilities of initiative,teamwork,and coordination in the postgraduates,and the requirements for postgraduate cultivation can also be satisfied.