健康献血者血浆可溶性糖蛋白A(GPA)在抗-M与抗-"Mia"抗体阳性及阴性中表达差异的分析研究
Analysis of the Difference of Plasma Soluble Glycoprotein A Expression in Positive and Negative Anti-M and Anti-"Mia"Levels in Healthy Blood Donors
梁延连 1邬林枫 1唐雄驰 2苏宇清 1吴凡 1梁爽 1孙丽艳1
作者信息
- 1. 深圳市血液中心输血医学研究所,广东深圳 518040
- 2. 广西平南县人民医院,广西平南 537300
- 折叠
摘要
目的 分析健康献血者血浆中可溶性糖蛋白A(glycoprotein A,GPA)表达量与抗-M及抗-"Mia"抗体的相关性.方法 收集2022年2月9日～2023年2月15日的健康献血者血浆:不规则抗体阴性的NN型(Ⅰ组,n=118)与MM型(Ⅱ组,n=51),抗-M抗体阳性的NN型(Ⅲ组,n=145)与抗-"Mia"抗体阳性的随机型(Ⅳ组,n=87),分别检测 4组人不同个体血浆中GPA的含量,t检验分析GPA表达量的差异.结果 Ⅰ组、Ⅱ组、Ⅲ组和Ⅳ组血浆中GPA平均含量分别为:9.941±0.252,10.97±0.256,5.139±0.129 和 4.28±0.139ng/ml.Ⅰ组与Ⅱ组的GPA平均含量较高,Ⅲ组与Ⅳ组的GPA平均含量较低,显示GPA平均含量在不规则抗体阴性(Ⅰ组与Ⅱ组)的血浆中较高,在抗-M与抗-"Mia"抗体阳性(Ⅲ与Ⅳ组)的血浆中较低,差异具有统计学意义(均P＜0.01).结论 含有抗-M与抗-"Mia"抗体的健康献血者血浆中GPA含量明显低于抗体阴性的群体.该研究结果为深入探讨血浆中GPA是否有中和抗-M与抗-"Mia"抗体的能力,提高疾病诊断与安全输血打下基础.
Abstract
Objective To analyze the correlation between the expression of soluble glycoprotein A(GPA)in plasma of healthy blood donors and anti-M and anti-"Mia"antibodies.Methods Plasma from healthy donors from February 9,2022 to February 15,2023 was collected:irregular antibody-negative NN type(group Ⅰ,n=118)and MM type(group Ⅱ,n=51),anti-M antibody positive NN type(group Ⅲ,n=145)and anti-"Mia"antibody positive companion type(group Ⅳ,n= 87),the GPA content in plasma of different individuals in 4 groups was detected,and the difference in GPA expression was analyzed by t-test.Results The average plasma GPA contents in groupsⅠ,Ⅱ,Ⅲ and Ⅳ were 9.941±0.252,10.97±0.256,5.139±0.129 and 4.28±0.139ng/ml,respectively.The average GPA content of groups Ⅰ and Ⅱ was higher,and the average GPA content of groups Ⅲ and Ⅳ was lower,and the differences were statistically significant(all P＜0.01).Conclusion The GPA content in plasma of healthy donors with anti-M and anti-"Mia"antibodies was significantly lower than that of the antibody-negative group.The results of this study lay a foundation for further investigation of whether GPA in plasma has the ability to neutralize anti-M and anti-"Mia"antibodies,improve disease diagnosis and safe blood transfusion.
关键词
糖蛋白A/MN血型/抗-M抗体/抗-"Mia"抗体Key words
glycoprotein A/MN blood group/anti-M antibody/anti-"Mia"antibody引用本文复制引用
基金项目
深圳市医学三名工程项目(SZSM201811092)
深圳市医学重点学科建设项目(SZXK070)
广西医疗卫生重点培育学科项目()
出版年
2024
国家科技期刊平台
NSTL
万方数据