自动化磁珠法提取血清脂溶性维生素应用LC-MS/MS检测的性能评价
Performance Evaluation of Automated Magnetic Beads Extraction Method for Extraction of Serum Fat-soluble Vitamins by LC-MS/MS Detection
孔卓 1李春艳1
作者信息
- 1. 北京积水潭医院医学检验中心,北京 100035
- 折叠
摘要
目的 评估自动化磁珠法提取血清脂溶性维生素应用液相色谱串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)检测的性能.方法 收集 200 例临床剩余血清样本,采用自动化磁珠法提取血清中脂溶性维生素A,D2,D3,E和K;同时联合LC-MS/MS检测脂溶性维生素A,D2,D3,E和K的线性、定量限、精密度、正确度、携带污染率等性能指标以及基质效应.并比较此方法与传统萃取法检测结果的一致性.结果 自动化磁珠法提取脂溶性维生素A,D2,D3,E和K线性相关系数均＞0.99;五种物质的定量限分别为 5,0.25,0.25,125 和 0.025 ng/ml;批内精密度和批间精密度分别为 0.66%～4.83%,0.15%～3.70%;平均加标回收率为 87.05%～111.11%;基质效应为 95.43%～99.07%;高-低值样本循环进样结果均值与低-低值样本循环进样结果均值之差,均小于低-低值样本循环进样结果均值的 3s;统计学结果显示自动化磁珠法和传统萃取法提取的脂溶性维生素结果相关性良好(r＞0.99),两种方法的检测结果无显著偏倚.结论 自动化磁珠法提取脂溶性维生素的检测性能良好,有望提高样品通量和分析效率.
Abstract
Objective To evaluate the performance of the automated magnetic beads extraction method for extraction of serum fat-soluble vitamins by liquid chromatography-tandem mass spectrometry(LC-MS/MS).Methods A total of 120 clinical residual serum samples were collected automated magnetic beads extraction method was applied to extract serum fat-soluble vitamins A,D2,D3,E and K.The linearity,limit of quantification,precision,accuracy,carryover and matrix effect of fat-soluble vitamins A,D2,D3,E and K were detected by LC-MS/MS.The consistency of the method and conventional extraction method was compared.Results The linear correlation coefficients for extracting fat-soluble vitamins A,D2,D3,E and K by automated magnetic beads extraction method were all greater than 0.99,and the quantitative limits for these five fat-soluble vitamins were 5,0.25,0.25,125 and 0.025 ng/ml.The intra-batch precision and inter-batch precision were 0.66%～4.83%and 0.15%～3.70%,respectively,and the average spike recovery rate was 87.05%～111.11%.The matrix effect was 95.43%～99.07%,and the difference between the mean value of the cyclic injection results of high-low value samples and the mean value of the cyclic injection results of low-low value samples was less than 3s of the mean value of the cyclic injection results of low-low value samples.The statistical analysis showed that the correlation between the results of the automated magnetic beads extraction method and the conventional extraction method was good(r＞0.99),and there was no significant bias in the detection results of the two methods.Conclusion The automated magnetic beads extraction method for extraction of fat-soluble vitamins had good detection performance,which may be expected to improve sample flux and analysis efficiency.
关键词
自动化磁珠法/液相色谱串联质谱/脂溶性维生素Key words
automated magnetic beads extraction method/liquid chromatography tandem mass spectrometry/fat-soluble vitamins引用本文复制引用
基金项目
国家自然科学基金青年科学基金(82100257)
北京积水潭医院医工结合项目(YGQ-202311)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据