内源性因素对抗体夹心免疫法检测血清心肌肌钙蛋白I的干扰及解决方案研究进展
Research Progress on Interference of Endogenous Factors in Detection of Serum Cardiac Troponin I by Sandwich Antibody Immunoassay and Its Solutions
何成山 1刘洋 2徐正 1蒋秀娣 1陆志成1
作者信息
- 1. 上海中医药大学附属第七人民医院医学检验科,上海 200137
- 2. 上海中医药大学,上海 201203
- 折叠
摘要
心肌肌钙蛋白I(cardiac troponin I,cTnI)是临床上诊断心肌损伤的首选血清学标志物.cTnI检测基于抗体夹心免疫法,不同检测试剂中检测和捕获抗体所针对的cTnI抗原表位不一致,易造成cTnI检测结果的异质性.内源性干扰因素如cTnI自身抗体、异嗜性抗体、类风湿因子等可严重的干扰cTnI检测结果,影响临床上对心肌损伤类疾病的诊断、治疗及预后判断.该文就cTnI的抗体夹心免疫检测和内源性因素对cTnI检测的干扰及解决方案等方面研究进展作一综述,为临床上鉴别诊断cTnI异常检测结果提供理论依据.
Abstract
Cardial troponin I(cTnI)is the preferred serological marker for the diagnosis of myocardial injury.cTnI detection is based on antibody sandwich immunoassay.The epitopes of cTnI antigen targeted by detecting and capturing antibodies in different detection reagents are inconsistent,which easily leads to the heterogeneity of cTnI detection results.Endogenous interfering factors such as cTnI autoantibody,heterophile antibody,rheumatoid factor,ect,which can seriously interfere with the results of cTnI detection,and affecting the clinical diagnosis,treatment and prognosis of myocardial injury diseases.In this paper,the research progress of antibody sandwich immunoassay for cTnI and interference of endogenous factors on cTnI detection and solutions are reviewed to provide theoretical basis for differential diagnosis of abnormal cTnI detection results in clinical practice.
关键词
心肌肌钙蛋白I/抗体/夹心免疫/干扰/内源性因素Key words
cardiac troponin I/antibodies/sandwich immunoassay/interference/endogenous factor引用本文复制引用
基金项目
上海市卫生健康委科研面上项目(202040165)
浦东新区卫生健康委公共卫生学科项目(PWGw2020-02)
出版年
2024
国家科技期刊平台
NSTL
万方数据