现代检验医学杂志2024，Vol.39Issue(3) ：67-71,182.DOI:10.3969/j.issn.1671-7414.2024.03.011

高血压并发冠状动脉粥样硬化性心脏病患者血清miR-27a-3p和HOXC6水平表达与冠状动脉病变的相关性研究

Correlation between Serum miR-27a-3p and HOXC6 Levels and Coronary Artery Disease in Patients with Hypertension Complicated with Coronary Atherosclerotic Heart Disease

孙实 李想
现代检验医学杂志2024，Vol.39Issue(3) ：67-71,182.DOI:10.3969/j.issn.1671-7414.2024.03.011
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高血压并发冠状动脉粥样硬化性心脏病患者血清miR-27a-3p和HOXC6水平表达与冠状动脉病变的相关性研究

Correlation between Serum miR-27a-3p and HOXC6 Levels and Coronary Artery Disease in Patients with Hypertension Complicated with Coronary Atherosclerotic Heart Disease

孙实 1李想2
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作者信息

  • 1. 北大荒集团总医院 心内科,哈尔滨 150088
  • 2. 北大荒集团总医院 病理科,哈尔滨 150088
  • 折叠

摘要

目的 探讨高血压并发冠状动脉粥样硬化性心脏病(冠心病)患者血清微小核糖核酸-27a-3p(miR-27a-3p)和同源异型盒C6(homeobox C6,HOXC6)水平表达与冠状动脉病变的相关性,为高血压并发冠心病的防治提供一定参考.方法 选取北大荒集团总医院2021年6月～2023年6月接诊的246例高血压患者为研究对象,其中129例高血压并发冠心病患者作为并发组,117例单纯高血压患者作为未并发组.采用实时荧光定量PCR(quantitative real time PCR,qRT-PCR)检测血清miR-27a-3p和HOXC6表达水平;运用Spearmian法分析高血压并发冠心病患者血清miR-27a-3p和HOXC6水平与冠状动脉病变的相关性;多因素Logistic回归分析高血压并发冠心病的影响因素;受试者工作特征(receiver operating characteristic,ROC)曲线分析血清miR-27a-3p,HOXC6水平对高血压并发冠心病的预测价值.结果 并发组高血压病程(6.69±1.35年)、总胆固醇(total cholesterol,TC)(5.96±0.86mmol/L)、低密度脂蛋白-胆固醇(low-density lipoprotein cholesterol,LDL-C)(3.36±0.51mmol/L)均显著高于未并发组(5.87±1.32 年,5.42±0.67mmol/L,2.98±0.48mmol/L),并发组血清 miR-27a-3p(1.35±0.34),HOXC6(1.29±0.33)水平均显著高于未并发组(1.06±0.23,1.03±0.25),差异具有统计学意义(t=4.808,5.454,6.001,7.755,6.911,均 P＜0.05);轻度组、中度组、重度组患者血清 miR-27a-3p(0.54±0.13,1.19±0.31,2.18±0.55)和 HOXC6(0.47±0.12,1.12±0.31,2.13±0.52)表达水平逐渐升高,差异具有统计学意义(F=191.026,191.026,均P＜0.05).高血压并发冠心病患者血清miR-27a-3p,HOXC6与SYNTAX评分具呈正相关性(r=0.563,0.517,均P＜0.05);血清miR-27a-3p[OR(95％CI:1.627(1.177～2.248)]、HOXC6[OR(95％CI):1.715(1.069～2.750)]是影响高血压并发冠心病的独立危险因素(P＜0.05);血清miR-27a-3p和HOXC6联合预测高血压并发冠心病的AUC为0.896(95％CI:0.851～0.931),优于各自单独诊断(Z=2.133,2.815,均P＜0.05).结论 高血压并发冠心病患者血清miR-27a-3p,HOXC6水平均显著升高,且与冠状动脉病变密切相关,二者联合对高血压并发冠心病具有较高的预测价值.

Abstract

Objective To investigate the correlation between serum microRNA(miR)-27a-3p and homeobox C6(HOXC6)levels expression and coronary artery disease in patients with hypertension complicated by coronary atherosclerotic heart disease(CHD),and provide some references for the prevention and treatment of hypertension complicated with coronary heart disease.Methods A total of 246 hypertensive patients accepted by Beidahuang Group General Hospital from June 2021 to June 2023 were collected as the study subjects,with 129 hypertensive patients complicated with coronary heart disease as the concurrent group and 117 simple hypertensive patients as the non concurrent group.Quantitative real time fluorescent PCR(qRT-PCR)was applied to detect the expression levels of miR-27a-3p and HOXC6 in serum.Spearman method was applied to analyze the correlation between serum miR-27a-3p and HOXC6 levels and coronary artery disease in hypertensive patients with coronary heart disease.Multivariate logistic regression was applied to analyze the influencing factors of hypertension complicated with coronary heart disease.Receiver operating characteristic(ROC)curve was applied to analyze the predictive value of serum miR-27a-3p and HOXC6 levels for hypertension complicated with coronary heart disease.Results The course of hypertension(6.69±1.35年),total cholesterol(TC)(5.96±0.86mmol/L)and low-density lipoprotein cholesterol(LDL-C)(3.36±0.51mmol/L)in the concurrent group were higher than those in the non concurrent group(5.87±1.32 年,5.42±0.67mmol/L,2.98±0.48mmol/L),the serum levels ofmiR-27a-3p(1.35±0.34)andHOXC6(1.29±0.33)in the concurrent group were higher than those in the non concurrent group(1.06±0.23,1.03±0.25),and the differences were statistically significant(t=4.808,5.454,6.001,7.755,6.911,all P＜0.05).The expression levels of miR-27a-3p(0.54±0.13,1.19±0.31,2.18±0.55)and HOXC6(0.47±0.12,1.12±0.31,2.13±0.52)in mild,moderate and severe groups were gradually increased,and the differences were significant(F=191.026,191.026,all P＜0.05).There were positive correlations between serum miR-27a-3p,HOXC6,and SYNTAX scores in patients with hypertension complicated by coronary heart disease(r=0.563,0.517,all P＜0.05).Serum miR-27a-3p[OR(95％CI):1.627(1.177～2.248)]and HOXC6[OR(95％CI):1.715(1.069～2.750)]were independent risk factors for hypertension complicated with coronary heart disease(all P＜0.05).The combination of miR-27a-3p and HOXC6 levels had an area under the curve(AUC)of 0.896(95％CI:0.851～0.931)for predicting hypertension complicated with coronary heart disease,which was superior to their respective individual diagnoses(Z=2.133,2.815,all P＜0.05).Conclusion The serum levels of miR-27a-3p and HOXC6 in patients with hypertension complicated by coronary heart disease were increased,which was closely related to coronary artery disease.The combination of the two has a high predictive value for hypertension complicated with coronary heart disease.

关键词

微小核糖核酸-27a-3p/同源异型盒C6/高血压/冠状动脉粥样硬化性心脏病/冠状动脉病变

Key words

micro RNA-27a-3p/homeobox C6/hypertension/coronary atherosclerotic heart disease/coronary artery disease

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出版年

2024
现代检验医学杂志
陕西省临床检验中心,陕西省人民医院

现代检验医学杂志

CSTPCD
影响因子：0.713
ISSN：1671-7414
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