妊娠期糖尿病患者血清LncRNA DANCR和miR-33a-5p水平表达对妊娠结局预测价值研究
Study on the Predictive Value of Serum LncRNA DANCR and miR-33a-5p Levels in Patients with Gestational Diabetes Mellitus on Pregnancy Outcome
王春燕 1刘慧赏 1马少未1
作者信息
- 1. 衡水市第四人民医院妇产科,河北衡水 053000
- 折叠
摘要
目的 探讨妊娠期糖尿病(gestational diabetes mellitus,GDM)患者血清长链非编码核糖核酸分化拮抗非蛋白编码 RNA(long noncoding RNA differentiation antagonizing nonprotein coding RNA,LncRNA DANCR)、微小 RNA-33a-5p(miR-33a-5p)水平表达对妊娠结局的预测价值.方法 选取2021年8月～2023年2月于衡水市第四人民医院产检和分娩的154例GDM患者为GDM组,并根据妊娠结局分为良好结局组(n=115)和不良结局组(n=39);同期收集24周葡萄糖耐量试验正常的149例健康孕产妇作为对照组.采用实时荧光定量PCR(qRT-PCR)检测血清LncRNA DANCR 和 miR-33a-5p 水平;采用受试者工作特征(receiver operating characteristic,ROC)曲线分析血清 LncRNA DANCR和miR-33a-5p预测GDM患者不良妊娠结局的价值;采用Pearson相关性分析GDM不良妊娠结局患者血清LncRNA DANCR,miR-33a-5p与空腹血糖(FBG)、空腹胰岛素(FINS)、稳态模式评估法计算胰岛素抵抗指数(HOMA-IR)的相关性;Logistic回归分析GDM患者不良妊娠结局的影响因素.结果 GDM组血清LncRNA DANCR水平(0.69±0.15)显著低于对照组(1.01±0.22),miR-33a-5p(1.59±0.40)及不良妊娠结局总发生率(25.34％)显著高于对照组(1.02±0.23,5.36％),差异具有统计学意义(t/x2=14.835,15.140,23.011,均P＜0.05).不良妊娠结局组血清 LncRNADANCR 水平(0.50±0.14)显著低于良好结局组(0.75±0.18),miR-33a-5p(2.00±0.58),FBG(8.97±0.66mmol/L),FINS(18.63±1.31pmol/ml)和 HOMAIR(7.42±0.98)显著高于良好结局组(1.45±0.26,8.01±0.59mmol/L,14.32±1.29pmol/ml,5.10±0.86),差异具有统计学意义(t=7.895～17.961,均P＜0.05).LncRNA DANCR,miR-33a-5p单独及二者联合预测GDM患者不良妊娠结局的曲线下面积(area under curve,AUC)分别为0.820,0.819和0.897.GDM不良妊娠结局患者血清LncRNA DANCR与FBG,FINS,HOMA-IR呈负相关(r=-0.498,-0.513,-0.509,均P＜0.05),miR-33a-5p 与 FBG,FINS,HOMA-IR 呈正相关(r=0.517,0.494,0.507,均 P＜0.05).LncRNA DANCR 是影响 GDM 患者不良妊娠结局的保护因素(OR=0.804,95％CI:0.693～0.933,P=0.004),miR-33a-5p是影响GDM患者不良妊娠结局的危险因素(OR=2.747,95％CI:1.444～5.225,P=0.002).结论 GDM不良妊娠结局患者LncRNADANCR降低,miR-33a-5p升高,二者均是不良妊娠结局的影响因素.
Abstract
Objective To investigate predictive value of serum levels of long noncoding RNA differentiation antagonizes non-protein coding RNA(LncRNA DANCR)and microRNA(miR)-33a-5p in patients with gestational diabetes mellitus(GDM)on pregnancy outcome.Methods A total of 154 GDM patients who underwent prenatal examination and delivery in the Fourth People's Hospital of Hengshui from August 2021 to February 2023 were collected as the GDM group,and these patients were grouped into a good outcome group of 115 cases and an adverse outcome group of 39 cases based on pregnancy outcomes.Meantime,149 healthy pregnant women with normal glucose tolerance test for 24 weeks were collected as the control group.Realtime fluorescence quantitative PCR(qRT-PCR)method was applied to detect serum LncRNA DANCR and miR-33a-5p levels,and receiver operating characteristic(ROC)curve was used to analyze the value of serum LncRNA DANCR and miR-33a-5p in predicting adverse pregnancy outcomes in GDM patients.Pearson correlation was applied to analyze the correlation among serum LncRNA DANCR,miR-33a-5p,fasting bloodglucos(FBG),fasting insulin(FINS),and homeostasis model assessment of insulin resistance(HOMA-IR)in patients with GDM adverse pregnancy outcomes.Logistic regression was used to analyze the influencing factors of adverse pregnancy outcomes in GDM patients.Results The serum LncRNA DANCR level(0.69±0.15)in the GDM group was lower than that in the control group(1.01±0.22),while the level of miR-33a-5p(1.59±0.40)and the total incidence of adverse pregnancy outcomes(25.34％)were higher than those in the control group(1.02±0.23,5.36％),with significant differences(t/x2=14.835,15.140,23.011,all P＜0.05).The serum LncRNADANCR level(0.50±0.14)in the adverse outcome group was lower than that in the good outcome group(0.75±0.18),while miR-33a-5p(2.00±0.58),FBG(8.97±0.66mmol/L),FINS(18.63±1.31pmol/ml)and HOMA-IR(7.42±0.98)were higher than those in the good outcome group(1.45±0.26,8.01±0.59mmol/L,14.32±1.29pmol/ml,5.10±0.86),the differences were statistically significant(t=7.895～17.961,all P＜0.05).The areas under curve(AUC)of predicting adverse pregnancy outcomes in GDM patients with LncRNA DANCR and miR-33a-5p alone and in combination were 0.820,0.819 and 0.897,respectively.For patients with GDM adverse pregnancy outcomes,serum LncRNA DANCR was negatively correlated with FBG,FINS and HOMAIR(r=-0.498,-0.513,-0.509,allP＜0.05),while miR-33a-5p was positively correlated with FBG,FINS and HOMA-IR(r=0.517,0.494,0.507,all P＜0.05).LncRNA DANCR was a protective factor that affected adverse pregnancy outcomes in GDM patients(OR=0.804,95％CI:0.693～0.933,P=0.004);while miR-33a-5p was a risk factor for adverse pregnancy outcomes in GDM patients(OR=2.747,95％CI:1.444～5.225,P=0.002).Conclusion LncRNA DANCR was decreased and miR-33a-5p was increased in GDM patients with adverse pregnancy outcomes,indicating both may be influencing factors for adverse pregnancy outcomes.
关键词
妊娠期糖尿病/妊娠结局/长链非编码核糖核酸分化拮抗非蛋白编码RNA/微小RNA-33a-5pKey words
gestational diabetes mellitus/pregnancy outcome/long noncoding RNA differentiation antagonizes nonprotein coding RNA/miR-33a-5p引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据