Relationship between the Expression of Serum miR-216b and miR-132 Levels and Clinical Prognosis in Patients with Gastric Cancer
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国家科技期刊平台
NETL
NSTL
万方数据
目的 探讨胃癌患者血清miR-216b和miR-132水平表达与临床预后的关系.方法 选取2018年1月～2020年2月在佳木斯市中心医院就诊的87例胃癌患者作为胃癌组,选择同期87例健康体检者作为对照组.实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)检测血清中 miR-216b 和 miR-132 表达水平,分析胃癌患者血清 miR-216b和miR-132表达水平与临床病理特征的关系.根据胃癌患者随访期间的生存或死亡情况,将胃癌患者分为预后良好组(生存,n=51)和预后不良组(死亡,n=36),用受试者工作特征(receiver operating characteristic,ROC)曲线分析血清miR-216b和miR-132表达水平对胃癌患者预后的预测价值;COX回归分析影响胃癌患者预后的因素.结果 与对照组比较,胃癌组血清中 miR-216b(0.69±0.20 vs 1.02±0.24)和 miR-132(0.73±0.19 vs 1.01±0.22)表达水平降低,差异具有统计学意义(t=9.853,8.984,均P＜0.001).分化程度为低分化、TNM分期为Ⅲ±Ⅳ期、有淋巴结转移、有远处转移的胃癌患者血清miR-216b,miR-132表达水平低于分化程度为中、高分化、TNM分期为Ⅰ±Ⅱ期、无淋巴结转移、无远处转移的胃癌患者,差异具有统计学意义(t=6.266,3.412,2.890,2.723;4.999,3.734,4.180,5.502,均P＜0.05).与预后良好组比较,预后不良组胃癌患者血清中miR-216b(0.56±0.16 vs 0.78±0.23)和miR-132(0.60±0.11 vs 0.82±0.25)表达水平降低,差异具有统计学意义(t=4.952,4.946,均 P＜0.001).血清 miR-216b,miR-132及二者联合预测胃癌患者预后的曲线下面积(area under the cure,AUC)分别为0.797(95％CI:0.706～0.889),0.832(95％CI:0.745～0.918)和 0.900(95％CI:0.836～0.964);敏感度和特异度分别为 83.3％,68.6％;97.2％,62.7％和79.4％,72.5％;当血清miR-216b,miR-132预测胃癌患者预后不良的截断值分别为0.66,0.76时,预测的敏感度较高.COX回归分析显示,miR-216b低表达、miR-132低表达、分化程度为低分化、TNM分期为Ⅲ+Ⅳ期、有淋巴结转移、有远处转移是胃癌患者预后不良的危险因素(均P＜0.05).结论 miR-216b和miR-132在胃癌患者血清中呈低表达,二者可作为预测胃癌患者预后的有效生物标志物.
Objective To investigate the relationship between serum microRNAs(miRNAs,miR)-216b and miR-132 expressions and clinical prognosis in patients with gastric cancer.Methods From January 2018 to February 2020,87 gastric cancer patients who visited Jiamusi Central Hospital were selected as the gastric cancer group,while 87 healthy individuals who underwent physical examinations in the hospital were collected as the control group.Quantitative real-time PCR(qRT-PCR)was applied to detect the expression levels of miR-216b and miR-132 in serum.The relationship between the expression levels ofmiR-216b and miR-132 in the serum of gastric cancer patients and their clinical pathological characteristics was analyzed.According to the survival or death status of gastric cancer patients during the follow-up period,these patients were divided into a good prognosis group(survival,n=51)and a poor prognosis group(death,n=36).The predictive value of serum miR-216b and miR-132 expression levels on the prognosis of gastric cancer patients was analyzed using the receiver operating characteristic(ROC)curve.COX regression was applied to analyze factors affecting the prognosis of gastric cancer patients.Results Compared with the control group,the expression levels of miR-216b(0.69±0.20vs1.02±0.24)and miR-132(0.73±0.19 vs 1.01±0.22)in serum of gastric cancer group were decreased,and the differences were significant(t=9.853,8.984,all P＜0.001).The expression levels of miR-216b and miR-132 in serum of gastric cancer patients with low differentiation,TNM stages Ⅲ±Ⅳ,lymph node metastasis,and distant metastasis were significantly lower than those of gastric cancer patients with medium and high differentiation,TNM stages Ⅰ±Ⅱ,no lymph node metastasis and no distant metastasis,and the differences were statistically significant(t=6.266,3.412,2.890,2.723;4.999,3.734,4.180,5.502,all P＜0.05).Compared with the good prognosis group,the expression levels of miR-216b(0.56±0.16 vs 0.78±0.23)and miR-132(0.60±0.11 vs 0.82±0.25)in serum of gastric cancer patients in the poor prognosis group were decreased,and the differences were statistically significant(t=4.952,4.946,all P＜0.001).The areas under the cure(AUC)of serum miR-216b,miR-132 and their combination predicted the prognosis of gastric cancer patients was 0.797(95％CI:0.706～0.889),0.832(95％CI:0.745～0.918)and 0.900(95％CI:0.836～0.964),respectively.The sensitivity and specificity were 83.3％,68.6％;97.2％,62.7％and 79.4％,72.5％,respectively.When the cut-off values of serum miR-216b and miR-132 for predicting poor prognosis of gastric cancer patients were 0.66 and 0.76,respectively,the sensitivity of the prediction were relatively high.COX regression analysis showed that low expression of miR-216b and miR-132,poorly differentiated degree,TNM stage Ⅲ+Ⅳ,lymph node metastasis,and distant metastasis were risk factors for poor prognosis in gastric cancer patients(all P＜0.05).Conclusion MiR-216b and miR-132 were low expressed in the serum of gastric cancer patients,and they could serve as effective biomarkers for predicting the prognosis of gastric cancer patients.
国家科技期刊平台
NSTL
万方数据
