现代检验医学杂志2024，Vol.39Issue(3) ：164-169.DOI:10.3969/j.issn.1671-7414.2024.03.028

糖尿病肾病患者血清Maresin1和PPBP水平表达与远期预后的相关性研究

Correlation between the Expression of Serum Maresin1 and PPBP Levels and Long Term Prognosis in Patients with Diabetes Nephropathy

宋江南 才蕊 张丹丹 孟斌 田明杰
现代检验医学杂志2024，Vol.39Issue(3) ：164-169.DOI:10.3969/j.issn.1671-7414.2024.03.028
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糖尿病肾病患者血清Maresin1和PPBP水平表达与远期预后的相关性研究

Correlation between the Expression of Serum Maresin1 and PPBP Levels and Long Term Prognosis in Patients with Diabetes Nephropathy

宋江南 1才蕊 2张丹丹 2孟斌 3田明杰1
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作者信息

  • 1. 唐山中心医院 临床营养科,河北唐山 063000
  • 2. 唐山中心医院 内分泌科,河北唐山 063000
  • 3. 唐山市工人医院内分泌科,河北唐山 063000
  • 折叠

摘要

目的 探讨糖尿病肾病(diabetes nephropathy,DN)患者血清Maresin1和前血小板碱性蛋白(pro-platelet basic protein,PPBP)表达水平及与远期预后的相关性.方法 选取2018年5月～2020年5月唐山市中心医院收治的83例糖尿病肾病患者为糖尿病肾病组,并选取同期60例单纯2型糖尿病患者为糖尿病组和60例健康体检者为对照组.采用酶联免疫吸附法(ELISA)检测血清Maresin1和PPBP水平,Spearman相关性分析血清Maresin1,PPBP与肾病理损伤的相关性,COX比例风险回归分析糖尿病肾病患者远期预后不良影响因素,受试者工作特征(ROC)曲线评估血清Maresin1和PPBP对远期预后不良的预测价值.结果 对照组、糖尿病组和糖尿病肾病组血清Maresin1水平(15.90±4.53ng/ml,12.34±4.29ng/ml,9.65±4.38ng/ml)依次降低,血清 PPBP 水平(263.45±85.22pg/ml,349.28±80.49pg/ml,435.76±87.21pg/ml)依次升高,差异具有统计学意义(F=35.159,72.678,均 P＜0.05).随着IFTA评分、间质炎症评分、肾小球分级的增加血清Maresin1水平降低(F=25.838,25.187,9.751,均P＜0.05),血清PPBP水平升高(F=56.513,92.702,58.137,均P＜0.05),差异具有统计学意义.血清Maresin1与IFTA评分、间质炎症评分、肾小球分级呈负相关(r=-0.637,-0.581,-0.594,均P＜0.05),血清PPBP与IFTA评分、间质炎症评分、肾小球分级呈正相关(r=0.659,0.664,0.608,均 P＜0.05).糖尿病肾病病程(HR=1.135,95％CI:1.012～1.370)、24h 尿蛋白(HR=1.087,95％CI:1.016～1.164)、PPBP(HR=1.208,95％CI:1.119～1.365)、IFTA 评分(HR=1.139,95％CI:1.024～1.219)、间质炎症评分(HR=1.122,95％CI:1.006～1.249)和肾小球分级(HR=1.139,95％CI:1.052～1.273)是糖尿病肾病患者远期预后的独立危险因素;eGFR(HR=0.934,95％CI:0.892～0.993)和Maresin1(HR=0.903,95％CI:0.816～0.982)是远期预后的保护因素(均P＜0.05).血清Maresin1,PPBP及两指标联合预测糖尿病肾病患者远期预后不良的曲线下面积(AUC)分别为0.781,0.777和0.901,两指标联合预测的AUC高于单项指标预测,差异具有统计学意义(Z=3.049,3.258,均P＜0.05).结论 糖尿病肾病患者血清Maresin1水平降低,PPBP水平升高,两指标与肾损伤程度密切相关,联合检测可有效预测患者远期不良预后.

Abstract

Objective To investigate the expression of serum Maresin 1 and pro-platelet basic protein(PPBP)in patients with diabetes nephropathy(DN)and their correlation with long-term prognosis.Methods A total of 83 patients with diabetes nephropathy admitted to Tangshan Central Hospital from May 2018 to May 2020 were selected as the diabetes nephropathy group.In the same period,60 patients with simple type 2 diabetes were selected as the diabetes group and 60 healthy people as the control group.Enzyme linked immunosorbent assay(ELISA)was used to detect the levels of serum Maresin1 and PPBP.Spearman correlation analysis was used to analyze the correlation between the levels of serum Maresin1 and PPBP and renal pathological damage.COX proportional hazard regression analysis was used to analyze the factors influencing the long-term poor prognosis of patients with diabetes nephropathy.ROC curve was used to evaluate the predictive value of serum Maresin1 and PPBP for the long-term poor prognosis.Results The levels of serum Maresin1(15.90±4.53 ng/ml,12.34±4.29 ng/ml,9.65±4.38 ng/ml)in the control group,diabetes group and diabetes nephropathy group were decreased in turn while the levels of serum PPBP(263.45±85.22 pg/ml,349.28±80.49 pg/ml,435.76±87.21 pg/ml)were increased in turn,and the differences were statistically significant(F=35.159,72.678,all P＜0.05).With the increase of IFTA score,interstitial inflammation score,and glomerular grading,serum Maresin1 level was decreased(F=25.838,25.187,9.751,all P＜0.05),while serum PPBP level was increased(F=56.513,92.702,58.137,all P＜0.05),and the differences were statistically significant,respectively.Serum Maresin1 was negatively correlated with IFTA score,interstitial inflammation score,and glomerular grading(r=-0.637,-0.581,-0.594,all P＜0.05),while serum PPBP was positively correlated with IFTA score,interstitial inflammation score,and glomerular grading(r=0.659,0.664,0.608,all P＜0.05),with significant differences.The course of diabetes nephropathy(HR=1.135,95％CI:1.012～1.370),24-hour urinary protein(HR=1.087,95％CI:1.016～1.164),PPBP(HR=1.208,95％CI:1.119～1.365),and IFTA score(HR=1.139,95％CI:1.024～1.219),interstitial inflammation score(HR=1.122,95％CI:1.006～1.249)and glomerular grading(HR=1.139,95％CI:1.052～1.273)were independent risk factors for long-term prognosis of diabetes nephropathy patients,while eGFR(HR=0.934,95％CI:0.892～0.993)and Maresin1(HR=0.903,95％CI:0.816～0.982)were protective factors for long-term prognosis(all P＜0.05).The areas under the curve(AUC)of serum Maresin1,PPBP and two indicators combined to predict the long-term poor prognosis of patients with diabetes nephropathy were 0.781,0.777 and 0.901,respectively.The AUC of two indicators combined was higher than that,and the differences were significant(Z=3.049,3.258,all P＜0.05).Conclusion In patients with diabetes nephropathy,serum Maresin1 was decreased and PPBP was increased,and the two indexes were closely related to the degree of renal injury.The combined test could effectively predict the long-term poor prognosis of patients.

关键词

糖尿病肾病/Maresin1/前血小板碱性蛋白

Key words

diabetic nephropathy/Maresin1/pro-platelet basic protein

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基金项目

河北省医学科学研究项目(20211274)

出版年

2024
现代检验医学杂志
陕西省临床检验中心,陕西省人民医院

现代检验医学杂志

CSTPCD
影响因子：0.713
ISSN：1671-7414
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