CRISPR-Cas13a系统在病原体检测应用中的最新研究进展
Recent Advances in the Application of CRISPR-Cas13a Systems in Pathogen Detection
李青松 1赵聪平 1刘静 1杨均 2燕思羽 1张银河3
作者信息
- 1. 西南医科大学公共卫生学院,四川泸州 646000
- 2. 泸州市人民医院,四川泸州 646000
- 3. 太极集团重庆涪陵制药厂有限责任公司,重庆 408000
- 折叠
摘要
规律成簇的间隔短回文重复序列及相关蛋白(CRISPR-Cas)系统已作为近年来兴起的新一代分子诊断工具,被广泛应用于基因编辑、核酸检测等领域,其中Cas13a亚型因其在核酸检测中高特异度、高灵敏度、无需昂贵设备、操作简单、费用低廉等特点,在病原体检测领域展现出巨大的潜力.该文综述了 CRISPR-Cas13a的作用机制、在病原体检测中的应用以及相关检测方法,并对Cas13a应用前景进行了展望.以期为CRISPR-Cas13a系统的进一步研究及其在病原体检测中的应用提供借鉴和参考.
Abstract
As a new generation of molecular diagnostic tools emerged in recent years,the clustered regularly interspaced short palindromic repeats/CRISPR-associated(CRISPR-Cas)systems have been widely used in gene editing,nucleic acid detection,and other fields.Due to its high specificity,high sensitivity,without expensive equipment,simple operation,and inexpensive characteristics in nucleic acid detection,the Cas13a subtype has shown great potential in the field of pathogen detection.This article reviews the mechanism of action of CRISPR-Cas13a,its application in pathogen detection,and related detection methods,and looks forward to the application prospects of Cas13a.To facilitate future investigations on the CRISPR-Cas13a systems and their potential in pathogen detection,this study aims to offer inspiration and serve as a valuable reference.
关键词
规律成簇的间隔短回文重复序列及相关蛋白13a系统/病原体/核酸检测Key words
clustered regularly interspaced short palindromic repeats/CRISPR-associated 13a systems/pathogen/nucleic acid detection引用本文复制引用
基金项目
西南医科大学应用基础项目(2021ZKQN002)
出版年
2024
国家科技期刊平台
NSTL
万方数据