Study on the Correlation between Serum miR-24-3p and MARK4 Level Expressions and Cardiac Function in Patients with Coronary Atherosclerotic Heart Disease
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维普
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目的 探讨动脉粥样硬化性心脏病(coronary atherosclerotic heart disease,CHD)患者血清中微小RNA(micro RNA,miR)-24-3p,微管亲和调节激酶 4(microtubule affinity-regulating kinase 4,MARK4)水平与心功能的相关性.方法 选取2021年8月～2023年4月间在四川省人民医院就诊的138例CHD患者为研究对象(CHD组),依据美国纽约心脏病学会(New York Heart Association,NYHA)分级,将 CHD 组患者分为 Ⅱ 级(n=39),Ⅲ级(n=68)和 Ⅳ级(n=31).另选取同期健康体检人员123例作为健康组.采用实时荧光定量PCR(qRT-PCR)法检测miR-24-3p及MARK4表达水平.检测CHD组及健康组人员心功能指标.采用Pearson法分析血清miR-24-3p,MARK4及与心功能指标间的相关性.结果 与健康组比较,CHD组患者的MARK4(1.19±0.21 vs 1.0 0±0.20),左心室收缩末期内径(LVESd)、左心室舒张末期内径(LVEDd)、室间隔厚度(IVS)、左室舒张末期容积(LVEDv)、左室收缩末期容积(LVESv)及左心室质量(LV mass)水平升高(t=7.462,18.242,23.888,9.941,2.812,12.520,11.029),miR-24-3p[(0.62±0.11)vs(1.00±0.17)],LVEF及心输出量(CO)水平降低(t=20.821,10.212,18.188),差异具有统计学意义(均P＜0.05).随着心功能分级加重,MARK4 表达水平(1.09±0.19,1.18±0.17,1.32±0.22),LVESd,LVEDd,IVS,LVEDv,LVESv 及 LV mass 逐渐升高(F=13.025,10.606,11.920,57.956,29.680,21.253,12.954),miR-24-3p表达水平(0.84±0.15,0.61±0.11,0.37±0.08),LVEF,CO水平逐渐降低(F=139.227,9.720,13.411),差异具有统计学意义(均P＜0.05).Pearson相关性分析显示,CHD组患者血清中miR-24-3p与MARK4表达呈负相关(r=-0.540,P＜0.05).CHD组患者血清miR-24-3p相对表达量与LVESd,LVEDd,IVS,LVEDv,LVESv 及 LV mass 呈负相关(r=-0.656,-0.636,-0.617,-0.576,-0.492,-0.687,均P＜0.05),与 LVEF 及 CO 呈正相关(r=0.570,0.683,均 P＜0.05);MARK4 相对表达量与 LVESd,LVEDd,IVS,LVEDv,LVESv 及 LVmass 呈正相关(r=0.503,0.542,0.508,0.624,0.516,0.560,均 P＜0.05),与 LVEF 及 CO 呈负相关(r=-0.594,-0.525,均P＜0.05).结论 CHD患者血清中miR-24-3p表达降低,MARK4表达升高,且二者与心功能指标间存在显著相关性.
Objective To investigate the correlation between serum microRNA(miR)-24-3p,microtubule affinity-regulating kinase 4(MARK4)level expression and cardiac function in serum of patients with coronary atherosclerotic heart disease(CHD).Methods From August 2021 to April 2023,138 CHD patients who visited Sichuan Provincial People's Hospital were collected as the study subjects(CHD group).According to the New York Heart Association(NYHA)grading,patients in the CHD group were separated into grade Ⅱ(n=39),grade Ⅲ(n=68),and grade Ⅳ(n=31).Additionally,123 individuals who underwent physical examinations were selected as the health group during the same period.Real-time fluorescence quantitative PCR(qRT-PCR)method was applied to detect the expression levels of miR-24-3p and MARK4.Cardiac function indicators in the CHD and health groups were detected.Pearson method was applied to analyze the correlation among serum miR-24-3p,MARK4,and cardiac function indicators.Results Compared with the healthy group,the levels of MARK4(1.19±0.21 vs 1.00±0.20),left ventricular end-systolic dimension(LVESd),left ventricular enddiastolic dimension(LVEDd),interventricular septal thickness(IVS),left ventricular end-diastolic volume(LVEDv),left ventricular end-systolic volume(LVESv),and left ventricular mass(LV mass)in the CHD group were increased(t=7.462,18.242,23.888,9.941,2.812,12.520,11.029),while the levels of miR-24-3p(0.62±0.11 vs 1.00±0.18),left ventricular ejection fraction(LVEF),and cardiac output(CO)were reduced(t=20.821,10.212,18.188),and the differences were statistically significant(all P＜0.05).As cardiac function grading increased,MARK4 expression levels(1.09±0.19,1.18±0.17,1.32±0.22),LVESd,LVEDd,IVS,LVEDv,LVESv and LV mass were increased(F=13.025,10.606,11.920,57.956,29.680,21.253,12.954),and miR-24-3p expression levels(0.84±0.15,0.61±0.11,0.37±0.08),LVEF and CO were decreased(F=139.227,9.720,13.411),and the differences were statistically significant(all P＜0.05).Pearson correlation analysis showed a negative correlation between miR-24-3p and MARK4 expression in the serum of patients with CHD(r=-0.540,P＜0.05).The relative expression of miR-24-3p in the serum of patients with CHD was negatively correlated with LVESd,LVEDd,IVS,LVEDv,LVESv and LV mass(r=-0.656,-0.636,-0.617,-0.576,-0.492,-0.687,all P＜0.05),but positively correlated with LVEF and CO(r=0.570,0.683,all P＜0.05).The relative expression level of MARK4 was positively correlated with LVESd,LVEDd,IVS,LVEDv,LVESv,and LV mass(r=0.503,0.542,0.508,0.624,0.516,0.560,all P＜0.05),but negatively correlated with LVEF and CO(r=-0.594,-0.525,all P＜0.05).Conclusion The expression of miR-24-3p in the serum of CHD patients was decreased,while the expression of MARK4 was increased.There was an obvious correlation between the two and cardiac function indicators.
coronary atherosclerotic heart diseasemicro RNA-24-3pmicrotubule affinity-regulating kinase 4cardiac function
维普
万方数据
