摘要
目的 探究老年膝关节骨性关节炎(knee osteoarthritis,KOA)患者血清长链非编码RNA(long non-coding RNA,LncRNA)膀胱癌相关转录因子 1(bladder cancer associated transcript 1,BLACAT1)和长链非编码 RNA(long non-coding RNA,LncRNA)-H19水平表达与病情严重程度的相关性.方法 收集2021年11月～2023年2月在荆门市中医医院进行诊治的120例老年KOA患者作为研究对象(KOA组).根据凯尔格伦-劳伦斯(Kellgren-Lawrence,K-L)分级将KOA组患者分为轻度组(n=41)、中度组(n=45)和重度组(n=34),另以同期行健康检查者为对照组(n=120).实时荧光定量PCR法测定血清LncRNA BLACAT1和LncRNA-H19水平,比较KOA组患者与对照组血清LncRNA BLACAT1和LncRNA-H19水平,分析不同病情程度KOA组患者血清LncRNA BLACAT1和LncRNA-H19水平.Spearman相关性分析KOA患者血清LncRNA BLACAT1和LncRNA-H19水平与病情程度间的关系,受试者工作特征(receiver operating characteristic,ROC)曲线分析血清 LncRNA BLACAT1 和 LncRNA-H19 水平对 KOA 的诊断价值.结果 KOA组患者血清LncRNA BLACAT1(1.31±0.31),LncRNA-H19(1.41±0.37)水平均显著高于健康对照组(0.99±0.24,1.03±0.25),差异具有统计学意义(t=8.941,9.322,均P＜0.05);重度组KOA患者血清LncRNA BLACAT1(1.53±0.36),LncRNA-H19(1.71±0.44)水平均显著高于轻度组(1.13±0.27,1.18±0.29)和中度组(1.32±0.31,1.40±0.38)(q=7.806,4.183;8.709,5.200,均 P＜0.05),且中度组高于轻度组(q=3.983,3.884,均 P＜0.05),差异具有统计学意义.Spearman相关性分析结果显示,血清LncRNA BLACAT1,LncRNA-H19水平与病情程度均呈显著正相关(r=0.552,0.414,均 P＜0.05).ROC 曲线结果显示,LncRNA BLACAT1,LncRNA-H19 单独诊断 KOA 的曲线下面积(area under the curve,AUC)分别为0.860,0.869,特异度分别为74.2％,80.8％,敏感度分别为66.7％,69.1％,两者联合诊断KOA的AUC为0.966,敏感度和特异度分别为90.0％,81.7％.结论 KOA患者血清LncRNA BLACAT1和LncRNA-H19水平均显著升高,且与KOA患者病情程度呈显著正相关,两者联合检测可有效预测KOA的发生.
Abstract
Objective To explore the correlation between the expression of serum long non-coding RNA bladder cancer associated transcript 1(LncRNA BLACAT1)and long non-coding RNA(LncRNA)-H19 levels and the severity of elderly knee osteoarthritis(KOA).Methods A total of 120 elderly KOA patients who underwent diagnosis and treatment in Jingmen Hospital of Traditional Chinese Medicine from November 2021 to February 2023 were selected as subjects(KOA group).Based on the Kelgren-Lawrence(K-L)classification,these KOA group patients were grouped into mild group(n=41),moderate group(n=45),and severe group(n=34).Meanwhile,those who underwent health examinations at the same time were regarded as control group(n=120).Real-time fluorescence quantitative PCR method was applied to measure the levels of serum LncRNA BLACAT1 and LncRNA-H19.The serum levels of LncRNA BLACAT1 and LncRNA-H19 were compared between KOA group and the control group.The serum levels of LncRNA BLACAT1 and LncRNA-H19 in KOA patients with different degrees of illness were analyzed.Spearman correlation was applied to analyze the relationship between the expression of serum LncRNA BLACAT1,LncRNA-H19 levels and the severity of KOA patients.Receiver operating characteristic(ROC)curve was applied to analyze the diagnostic value of serum LncRNA BLACAT1 and LncRNA-H19 levels for KOA.Results The serum levels of LncRNA BLACAT1(1.31±0.31)and LncRNA-H19(1.41±0.37)in KOA patients were higher than those in the healthy control group(0.99±0.24,1.03±0.25),and the differences were statistically significant(t=8.941,9.322,all P＜0.05).The serum levels of LncRNA BLACAT1(1.53±0.36)and LncRNA-H19(1.71±0.44)in the severe group were higher than those in the mild(1.13±0.27,1.18±0.29)and moderate groups(1.32±0.31,1.40±0.38)(q=7.806,4.183;8.709,5.200),and the serum levels of the moderate group were higher than that of the mild group(q=3.983,3.884),and the differences were statistically significant,respectively(all P＜0.05).Spearman correlation analysis results showed that the serum levels of LncRNA BLACAT1 and LncRNA-H19 were positively correlated with the severity of the disease(r=0.552,0.414,all P＜0.05).The results of ROC showed that the AUCs of LncRNA BLACAT1 and LncRNA-H19 alone diagnosed KOA were 0.860 and 0.869,with specificity of 74.2％and 80.8％,and sensitivity of 66.7％and 69.1％,respectively.The AUC of the combined diagnosis of KOA was 0.966,with a sensitivity and a specificity of 90.0％,81.7％,respectively.Conclusion The serum levels of LncRNA BLACAT 1 and LncRNA-H19 in KOA patients were elevated and were positively correlated with the severity of KOA patients.Combined detection of the two could effectively predict the occurrence of KOA.
维普
万方数据