As one of parasitic diseases,relevant reports of Lophomonas blattarum disease gradually increased since the first case in China.It is difficult to meet the clinical pathogen diagnosis requirements based solely on optical microscope morphological detection results as the diagnostic basis of Lophomonas blattarum disease.This study provides a review of the progress in laboratory diagnosis and detection of Lophomonas blattarum disease,aiming to establish more accurate and reliable pathogen detection methods for Lophomonas blattarum disease and to provide assistance for further research on Lophomonas blattarum disease.