Protective effects of silibinin on oxidative stress injury of H9c2 cardiomyocytes induced by alcohol
Objective To examine the protective effects of silibinin on oxidative stress damage of H9c2 cells induced by alcohol.Methods H9c2 cells were divided into a control group,an alcohol(500 mmol/L)group,and the silibinin(50 μmol/L,100 μmol/L)+alcohol(500 mmol/L)intervention group.The morphological change of H9c2 cells was observed under an optical microscope and cell survival was measured by CCK8 kit.Moreover,intracellular ROS content,mitochondrial membrane potential(MMP)changes,and apoptosis rates of H9c2 cells were tested by flow cytometry.In addition,lactate dehydrogenase(LDH)activity and malondialdehyde(MDA)content in culture medium,the activities of catalase(CAT),superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in cardiomyocytes were examined according to instructions.Results The morphology of H9c2 cells in the alcohol(500 mmol/L)group was abnormal and cell viability was decreased,the apoptosis rate was increased and the MMP was decreased,and the the irtracellular level of ROS was increased significantly.In addition,the LDH activity and MDA content in the culture medium were increased and the activities of antioxidant enzymes in cardiomyocytes were decreased,the differences were all statistically significant.Compared with the alcohol group,the morphology of H9c2 cells in the silibinin(100μmol/L)+alcohol(500 mmol/L)group was improved,and the survival rate was increased significantly.The apoptosis rate was decreased and the MMP was increased.In addition,the LDH activity and MDA content in culture medium were decreased and the activities of antioxidant enzymes in cardiomyocytes were increased significantly.The the irtracellular level of ROS was decreased.Conclusion H9c2 cells were induced by alcohol to construct oxidative stress injury model,silibinin pre-treated showed obvious protective effects on cell morphology,survival rate,apoptosis rate,and antioxidant enzyme activity.
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维普
