摘要
目的：探讨非小细胞肺癌 ERK、AKT 蛋白磷酸化的病理意义。方法：免疫组织化学 SP 法检测90例非小细胞肺癌的 p －ERK 和 p －AKT 表达状况，分析二者表达与通路上游 EGFR、KRAS、PI3K 表达及患者部分临床病理参数间的相关关系。结果：非小细胞肺癌中，p －ERK 表达阳性率为53．33％，其与患者的性别和吸烟史及肺腺癌的分化程度相关（P ＜0．05）。p －ERK 表达与 EGFR 及 KRAS 表达均无显著相关关系（P＞0．05）。p －AKT 表达阳性率为56．67％，p －AKT 表达与 EGFR 表达显著正相关（r ＝0．336，P ＜0．05），但与PI3K 表达无显著相关关系（P ＞0．05）。p －ERK 与 p －AKT 表达显著正相关（r ＝0．292，P ＜0．05）。结论：非小细胞肺癌中可能存在 ERK 的自身激活，检测 ERK 与 AKT 蛋白的磷酸化状态可能对筛选 EGFR －TKI 治疗获益人群有所帮助。
Abstract
Objective:To investigate the pathological significance of ERK and AKT phosphorylation in non -small cell lung cancer (NSCLC).Methods:Immunohistochemistry was used to detect the expression of p -ERK,p -AKT and their upstream proteins in 90 NSCLC samples,and the SPSS 13.0 statistic software was used to analyze the corre-lations of p -ERK and p -AKT protein expressions to patients'clinical pathological agents and their upstream protein expressions.Results:The positive rate of p -ERK protein expression in NSCLC was 53.33%,associating with gen-der,smoking status of patients and differentiation of the adenocarcinoma (P <0.05).While no significant relation-ships were found between p -ERK protein expression and EGFR,KRAS protein expressions (P >0.05).The positive rate of p -AKT protein expression in NSCLC was 56.67%.p -AKT protein expression was correlated with EGFR protein expression (r =0.336,P <0.05),but PI3K protein expression (P >0.05).p -ERK protein expression was correlated with p -AKT protein expression (r =0.292,P <0.05).Conclusion:The ERK protein might be activated spontaneously in NSCLC,and the detection of p -ERK and p -AKT protein expressions would be helpful to select pa-tients benefiting from EGFR -TKI therapy.
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