MiRNA-6507 enhances cisplatin sensitivity in cervical cancer cells and its mechanism exploration
Objective:Exploring the effect and mechanism of miRNA-6507 on cisplatin sensitivity in cervical cancer cells.Methods:The miRNA and clinical data related to cervical cancer were downloaded from the TCGA database to analyze the miRNA expression differences between adjacent and cancerous tissues,the miRNA related to patient sur-vival was further analysis.The expression difference of miRNA between normal cervical cells and cervical carcinoma cells was verified by RT-qPCR.The miRNA related to the 5-year survival rate of patients was chosen,and the miR-NA mimics and controls were transfected into cervical cancer cell lines.MTT was used to detect cell viability,and cell sensitivity to cisplatin was detected after cisplatin was added to transfected cells.Results:There were 256 tumor tis-sues and 2 para-cancer tissues in TCGA database.edgeR analysis showed that among 1 881 miRNA associated with cervical cancer,19 were up-regulated and 70 were down-regulated in tumor tissues compared with adjacent tissues.Each of the 89 miRNA were divided into high expression group and low expression group for survival analysis,results showed that miRNA-140,miRNA-145,miRNA-200c,and miRNA-6507 were correlated with the survival of pa-tients.The high expression of miRNA-140,miRNA-145 and miRNA-6507 can improve the survival of patients,and the high expression of miRNA-200c can reduce the survival of patients.RT-qPCR results showed that the ex-pression of miRNA-140 in cervical cancer cell lines Caski,HCC94 and Siha was significantly higher than that in cer-vical cancer cells H8,but there was no significant difference in C33A and Hela cells.The expression of miRNA-145 was decreased in all cervical cancer cells,which was consistent with survival analysis.The expression of miRNA-200c was up-regulated in Caski and HCC94 cervical cancer cells,which was the same as the results of survival anal-ysis,but there was no significant change in the expression of miRNA-200c in C33A,Hela and Siha cells.The expres-sion level of miRNA-6507 in the five cervical cancer cells verified was lower than that of normal cell line H8,which was consistent with survival analysis.MTT results showed that the cell viability of C33A and Siha after miRNA-6507 mimics transfection was not affected,and the cell viability of miRNA-6507 transfected cells after cisplatin treatment was decreased.Conclusion:miRNA-6507 can enhance the cisplatin sensitivity of cervical cancer cells and may be a candidate miRNA for cervical cancer treatment.
万方数据
维普
