Expression of high-temperature requirement factor A1 in medullary thyroid carcinoma tissues and its clinical significance
Objective:To evaluate the expression of high-temperature requirement factor A1(HTRA1)in medul-lary thyroid carcinoma(MTC)tissues and to explore its relationship with clinicopathological features,prognosis and tumor immunity.Methods:The expression of HTRA1 protein in 26 cases of MTC tissue was detected by immunohisto-chemistry.The correlations between HTRA1 protein expression and clinicopathological features and prognosis were analyzed.The relationship between HTRA1 expression and tumor immunity was revealed through bioinformatics.Results:HTRA1 proteins were mainly localized in the cytoplasm in MTC tissues,whereas they were mainly localized in the nucleus in normal thyroid tissues.HTRA1 protein expression was up-regulated in MTC tissues compared with normal thyroid tissues.Clinicopathological analysis showed that the expression of HTRA1 protein was associated with extrathyroidal extension,Hashimoto's thyroiditis and recurrence-free survival.The combined detection of HTRA1 pro-tein,preoperative serum calcitonin and carcinoembryonic antigen had a higher value in predicting extrathyroidal exten-sion,tumor size,tumor T stage and recurrence-free survival.Immunoinfiltration analysis showed that HTRA1 expres-sion was mainly related to immunosuppressive cells.The tumor immune dysfunction and exclusion(TIDE)algorithm revealed that HTRA1 expression in MTC was positively correlated with TIDE score,immune dysfunction,immune ex-clusion and cancer-associated fibroblast,while it was negatively correlated with microsatellite instability.Gene set enrichment analysis showed that in MTC,HTRA1 expression was mainly associated with gene sets of extracellular matrix and its related signaling pathways.Conclusion:HTRA1 may serve as a new therapeutic target for MTC.The association of HTRA1 with tumor immune function may be helpful for immunotherapy of MTC.
万方数据
维普
