Detection of Treg and Th17 level in peripheral blood of multiple myeloma and its clinical value
Objective:To detect Treg and Th17 in peripheral blood of patients with multiple myeloma and study their clinical value.Methods:Multiple myeloma patients(MM group)and normal healthy people(CON)were se-lected as research subjects.Peripheral blood Treg and Th17 were detected by flow cytometry,and Foxp3 and RORγt were detected by real-time fluorescence quantitative PCR(RT-qPCR).Compare the differences in Treg,Th17,Foxp3 and RORγt in different clinicopathological characteristics.The ROC curve was used to analyze the sensitivity and specificity of Treg combined with Th 17 in predicting poor 1-year prognosis in multiple myeloma.Multivariate Cox regression analysis of risk factors for death in multiple myeloma.Results:Treg,Th17,Foxp3 and RORγt in the MM group were higher than those in the CON normal healthy people,and the difference was statistically significant(P＜0.05).Treg,Th17,Foxp3 and RORγt in ISS stage Ⅲ,r-ISS stage Ⅲ,MRD+multiple myeloma patients were sig-nificantly higher than those in ISS stage Ⅰ to Ⅱ,r-ISS stage Ⅰ to Ⅱ,and MRD-patients with multiple myeloma(P＜0.05).The sensitivity,specificity and AUC of Treg combined with Th17 in predicting poor 1-year prognosis of multi-ple myeloma were higher than those of Treg,Th17,Foxp3,RORγt,ISS stage,r-ISS stage,and minimal residual disease(P＜0.05).Treg ≥8.23％,Th 17≥3.05％,Foxp3≥1.12,RORγt≥0.94,ISS stage Ⅲ,r-ISS stageⅢ,minimal residual disease MRD+are independent risk factors for death in patients with multiple myeloma(P＜0.05).Conclusion:Treg and Th 17 are significantly increased in patients with multiple myeloma,which are closely related to the condition and prognosis.They can be used as markers for the assessment of the condition and progno-sis of multiple myeloma.The combined detection of the two can significantly improve the prediction sensitivity and specificity of multiple myeloma.
万方数据
维普
