Research relating to Jiedu Xiaozheng yin inhibiting the expressions of EMT and PD-L1 of colorectal cancer cells
Objective:To approach the biological mechanism of Jiedu Xiaozheng yin(JXY)treating colorectal cancer(CRC)based on the perspective of epithelial-mesenchymal transition(EMT)and immune checkpoint ligand-1(PD-L1).Methods:The relevant data of 267 patients with CRC were collected from TCGA database.The correlativity between tumor-specific survival and EMT and PD-L1 of patients with CRC was analyzed by bioinformatics.The correlativity between EMT-related transcription factors and PD-L1 was analyzed by heat-map.The effect of JXY on the healing ability of CRC cells(HCT-15 and HCT-116)was detected by wound healing assay.The effect of JXY on the expressions of EMT-related factors ZEB 1,Snail,PD-L1,ZO-1 and E-cadherin etc.protein in both cell lines(CRC cells HCT-15 and HCT-116)was detected by Western blot assay.Results:High expressions of EMT and PD-L1 shortened the survival of patients with CRC significantly(EMT,P＜0.01;PD-L1,P＜0.05).The expression of PD-L1 gene was positively correlated to the expression of gene of EMT-related factors(TWIST1,TWIST2,ZEB 1,ZEB2,Snail1,Snail2,Vimentin,etc.),and high expression of PD-L1 was accompanied by high expression of EMT factor.The healing ability of JXY-intervened CRC cells was significantly decreased(P＜0.05).Meanwhile,JXY inhibited the expression of EMT factor-related proteins ZEB 1,Snail,PD-L1 in CRC cells,while up-regulated the expressions of ZO-1 and E-cadherin proteins.Conclusion:JXY inhibits the expressions of EMT and immune checkpoint ligand PD-L1 in colorectal cancer.Its inhibitory effect on colorectal cancer is related to anti-metastasis and regulation of immune microenvironment.
colorectal cancerJiedu Xiaozheng yinEMTPD-L1experimental research
万方数据
维普
