成簇的规律间隔的短回文重复序列(clustered regularly interspaced short palindromic repeats,简称为CRISPR)系统是一种新的基因修饰技术,具有制作周期短、成本低和作用高效等优点.本文从结构、研究历史、分类和分布、作用机制和其在生物学研究中的应用等方面介绍CRISPR-Cas系统.
Application of CRISPR-Cas System in Biology Research
Clustered regularly interspaced short palindromic repeats (CRISPR) system was a new genetic modification tool.Its advantages were short work time,low cost,high efficiency and so on.Here we review the CRISPR-Cas system structure,development history,classification,mechanism and application in biology research.