Preparation and Stability Validation of Nucleic Acid Samples for Genetic Quality Evaluation of Mice
Objective Two nucleic acid standard samples and primers of BALB/c and C57BL/6 were prepared for genetic quality evaluation of mice,and their integrity and stability were verified.Method Agarose gel electrophoresis and Ultraviolet spectrophotometry were used to determine the purity and concentration of mixed nucleic acid standard samples.And according to the sampling principle,the stability of storage and transportation were verified.The storage stability was verified by sampling at 4℃,room temperature(RT)and 37℃as the ordinate of observation,and 1,2,3,7 and 14 d as the abscissa of observation.An incubator,an ice bag and a thermometer were used to simulate the transportation conditions,and the transportation stability was verified for 1,3 and 7 days respectively.Result The A values of A260/A280 of BALB/c and C57BL/6 standard nucleic acid samples were between 1.7 and 1.9,and the average concentrations were 37.53 and 35.03 ng/μL,respectively.The stability test showed that the two standard nucleic acid samples and primers could be stably stored at 4℃and room temperature(RT)for 14 days,and could also be stably stored in the transportation environment for 7 days.Conclusion BALB/c and C57BL/6 nucleic acid samples have good integrity and stability,which can be used to evaluate the genetic quality of mice.
万方数据
维普
