Establishment of Type 2 Diabetes Model in Zebrafish
Objective The type 2 diabetic zebrafish model were constructed and evaluated in order to systematically study the occurrence and development mechanism of type 2 diabetes mellitus(T2DM).Method In this study,the two groups of wild-type blue zebrafish were kept in the same environment with the same light,food,water and other conditions.The zebrafish in the experimental group were immersed in 2%glucose solution every other day for 28 days to establish the zebrafish model of type 2 diabetes.The establishment of the model was evaluated by observing the morphological changes,drawing the weight curve,and biochemical detection of glucose and lipid metabolism.Result After 28 days,the weight gain curve of zebrafish in experimental group was significantly higher than that in control group.During the induction,the zebrafish in the experimental group showed behavioral changes,they swam faster,were more sensitive to environmental stimuli,and shed white metabolites,while control zebrafish kept normal swimming speed and shed brown metabolites.The eyes of the experimental group showed a red and swollen appearance.Meanwhile,the tissue glucose level,the tissue triglyceride level and the tissue total cholesterol level in the experimental group was significantly higher than that in the control group(P<0.05).Conclusion The zebrafish model of type 2 diabetes mellitus can be successfully established after 28 days of glucose immersion every other day.The pathogenic status of zebrafish can be evaluated by behavioral observation,weight gain curve drawing,and glucose and lipid biochemical detection.
万方数据
维普
