Determination of Biochemical Marker Genes in PVG Rats
Objective Biochemical marker genes were determined in PVG rats.Method According to the national standard GB/T 14927.1-2008"Biochemical Marker Detection Method for Inbred Mice and Rats in Laboratory Animals",4 PVG rats were randomly selected to collect tissues and organs(lung,kidney,small intestine,testis)for sample preparation.Eleven biochemical sites(Hbb,Cs1,Alp1,Akp1,Esl,Es3,Es4,Es6,Es8,Es9 and Esl0)were detected by acetate fiber film electrophoresis.Result The genotypes of 11 PVG rat loci were Hbb type b,Cs1 type b,Alp1 type b,Akp1 type a,Esl type a,Es3 type d,Es4 type b,Es6 type b,Es8 type b,Es9 type a and Esl0 type a,all of which were homozygous.Conclusion The result of genetic and biochemical markers in PVG rats were consistent with the characteristics of inbred lines.