摘要
目的 对 PVG大鼠进行生化标记基因的测定.方法 依据国家标准 GB/T 14927.1-2008《实验动物 近交系小鼠、大鼠生化标记检测法》测定,随机抽取 4 只 PVG大鼠,取(肺、肾、小肠、睾丸)组织器官进行样品制备,用醋酸纤维薄膜电泳法检测 11 个生化位点(Hbb、Cs1、Alp1、Akp1、Esl、Es3、Es4、Es6、Es8、Es9 和 Esl0).结果 PVG 大鼠 11 个位点的基因型分别是 Hbb为 b型,Cs1 为 b型,Alp1 为 b型,Akp1 为 a型,Esl为 a型,Es3 为 d型,Es4 为 b型,Es6 为 b型,Es8 为 b型,Es9 为 a型和 Esl0 为 a型,且均为纯合.结论 PVG 大鼠遗传生化标记结果符合近交系特征.
Abstract
Objective Biochemical marker genes were determined in PVG rats.Method According to the national standard GB/T 14927.1-2008"Biochemical Marker Detection Method for Inbred Mice and Rats in Laboratory Animals",4 PVG rats were randomly selected to collect tissues and organs(lung,kidney,small intestine,testis)for sample preparation.Eleven biochemical sites(Hbb,Cs1,Alp1,Akp1,Esl,Es3,Es4,Es6,Es8,Es9 and Esl0)were detected by acetate fiber film electrophoresis.Result The genotypes of 11 PVG rat loci were Hbb type b,Cs1 type b,Alp1 type b,Akp1 type a,Esl type a,Es3 type d,Es4 type b,Es6 type b,Es8 type b,Es9 type a and Esl0 type a,all of which were homozygous.Conclusion The result of genetic and biochemical markers in PVG rats were consistent with the characteristics of inbred lines.
维普
万方数据