Estrus Synchronization Using Progesterone P4 in Female Rats and Mice
Objective To explore the induction of estrus synchronization in SD rats and BALB/c mice females by progesterone injection.Method Progesterone hormone was injected into 8 weeks SD rats and BALB/c mice females on the first and second day of the experiment at doses of 10 mg/rat and 2 mg/mouse respectively,and cohabited on the fourth day.The observations of copulatory plugs were performed on the fifth,sixth and seventh day of the experiment.Embryo transfer was performed in copulatory plug checked pseudopregnant SD rats,and pseudopregnant rats obtained by mating with vasoligated males were used as controls.Blastocysts were flushed from embolized BALB/c mice females for embryonic stem(ES)cell injection.Result The highest copulatory plug rate of SD rats females injected with progesterone reached 44%on a single day and more than 80%overall.The highest copulatory plug rate of BALB/c mice females injected with progesterone reached 46%on a single day and more than 70%overall.The copulatory plug rate of progesterone injected group was 7-8 times higher than traditional method group on a single day,and more than 15 times higher than traditional method group overall.Compared with the traditional method,in progesterone injected group,the colony population of SD rats females and BALB/c mice females could be reduced to 10%-20%;the embryo birth rate of SD rats pseudopregnant could increase by about 13%.Conclusion Progesterone injection 10 mg/rat and 2 mg/mouse can be used for estrus synchronization in 8 weeks SD rats and BALB/c mice females respectively,and can significantly increase the copulatory plug rate and SD rats embryo birth rate.
万方数据
维普
