Objective To study the variation characteristics of gut microbiota of laboratory cats of different breeds and sexes by high-throughput sequencing of 16 S rDNA.Method A total of 21 laboratory cats were grouped according to the breed and sex of the laboratory cats,grouped by breed(ordinary group = 12,breed group = 9),grouped by sex(male group=9,female group=12).Fecal samples of cats were taken,DNA extraction,PCR amplification,and differential analysis of bacterial community structure and diversity by high-throughput sequencing of 16S rDNA.Result The dominant bacteria at the phylum in the experiment were Firmicutes,Bacteroides,and Actinobacteria(the sum of the relative abundances was more than 96%).The relatively abundant flora at the genus are Streptococcus、Enterococcus、Olsenella、Prevotella_9、Bifidobacterium and Collinsella.Sex can have an effect on the composition of the gut microbiota of laboratory cats,and there are statistically different abundances between male and female cats.Compared with female cats,Prevotella_9,Faecalibacterium and Terrisporobacter increased in the intestines of male cats(t test,P<0.05).Conclusion The basic characteristics of composition and abundance of the gut microbiota of laboratory cats are analyzed by 16S rDNA high-throughput sequencing,and the effects of sex and breed on the species composition of the gut microbiota of laboratory cats are described.
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