Analysis of Characteristics on Gut Microbiota of Laboratory Cats Based on 16S rDNA Sequencing
Objective To study the variation characteristics of gut microbiota of laboratory cats of different breeds and sexes by high-throughput sequencing of 16 S rDNA.Method A total of 21 laboratory cats were grouped according to the breed and sex of the laboratory cats,grouped by breed(ordinary group = 12,breed group = 9),grouped by sex(male group=9,female group=12).Fecal samples of cats were taken,DNA extraction,PCR amplification,and differential analysis of bacterial community structure and diversity by high-throughput sequencing of 16S rDNA.Result The dominant bacteria at the phylum in the experiment were Firmicutes,Bacteroides,and Actinobacteria(the sum of the relative abundances was more than 96%).The relatively abundant flora at the genus are Streptococcus、Enterococcus、Olsenella、Prevotella_9、Bifidobacterium and Collinsella.Sex can have an effect on the composition of the gut microbiota of laboratory cats,and there are statistically different abundances between male and female cats.Compared with female cats,Prevotella_9,Faecalibacterium and Terrisporobacter increased in the intestines of male cats(t test,P<0.05).Conclusion The basic characteristics of composition and abundance of the gut microbiota of laboratory cats are analyzed by 16S rDNA high-throughput sequencing,and the effects of sex and breed on the species composition of the gut microbiota of laboratory cats are described.
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