Objective The aim of this study was to investigate the genomic characteristics,virulence genes and antibiotic resistance genes of enteropathogenic Escherichia coli ILAREC-01 isolated from experimental rabbits with diarrhea.Method High-throughput sequencing was performed to determine the whole genome sequence of ILAREC-01.Eight databases including VFDB,ARDB,CAZy,SwisS-Prot,COG,CARD,KEGG and T3SS were used to annotate the function of the predict genes.In addition,the phylogenetic features of ILAREC-01 strain was also described.Result The genome of ILAREC-01 contains 5 249 genes.The analysis of the genome through CARD database showed that ILAREc-01 strain contains 59 resistance genes of 7 types of resistance mechanisms.Through VFDB database analysis,553 virulence genes were annotated in ILAREc-01.The Locus of enterocyte effacement pathogenicity island(LEE)was also identified in the genome of ILAREc-01.Phylogenetic trees analysis suggested that ILAREc-01 strain was highly homologous with FORC028、E2865 and 110512 strains.Conclusion In this study,the whole genome of rabbit enteropathogenic E.coli ILAREc-01 was sequenced,and the virulence genes,antibiotic resistance genes and evolution characteristics were also analyzed,which provided a reference for studying pathogenicity of enteropathogenic E.coli,and also provided data support for scientific prevention and control of colibacillosis in rabbits.
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