Whole Genome Sequencing Reveals the Distribution of Resistance and Virulence Genes of Enteropathogenic Escherichia Coli from Rabbits
Objective The aim of this study was to investigate the genomic characteristics,virulence genes and antibiotic resistance genes of enteropathogenic Escherichia coli ILAREC-01 isolated from experimental rabbits with diarrhea.Method High-throughput sequencing was performed to determine the whole genome sequence of ILAREC-01.Eight databases including VFDB,ARDB,CAZy,SwisS-Prot,COG,CARD,KEGG and T3SS were used to annotate the function of the predict genes.In addition,the phylogenetic features of ILAREC-01 strain was also described.Result The genome of ILAREC-01 contains 5 249 genes.The analysis of the genome through CARD database showed that ILAREc-01 strain contains 59 resistance genes of 7 types of resistance mechanisms.Through VFDB database analysis,553 virulence genes were annotated in ILAREc-01.The Locus of enterocyte effacement pathogenicity island(LEE)was also identified in the genome of ILAREc-01.Phylogenetic trees analysis suggested that ILAREc-01 strain was highly homologous with FORC028、E2865 and 110512 strains.Conclusion In this study,the whole genome of rabbit enteropathogenic E.coli ILAREc-01 was sequenced,and the virulence genes,antibiotic resistance genes and evolution characteristics were also analyzed,which provided a reference for studying pathogenicity of enteropathogenic E.coli,and also provided data support for scientific prevention and control of colibacillosis in rabbits.
万方数据
维普
