Effects of Nicorandil on Reendothelialization of Injured Artery from Animal Model of Balloon Injury in Rat Carotid Artery
Objective To investigate the effect of nicorandil on reendothelialization of injured artery from rat carotid artery balloon-injured model.Method A total of 18 Sprague-Dawley(SD)rats were randomly divided into 3 groups:sham operation group,control group and experimental group,with 6 rats in each group.The common carotid artery was deendothelialised in the control group and the experimental group,while only the common carotid artery was isolated in the sham operation group.After operation,the control group was given 0.9%sodium chloride solution(0.5 mL/100 g,once a day),the experimental group and sham operation group were given nicorandil(2 mg/100 g,once a day)by intragastric administration for 14 days,and common carotid artery samples were collected.The lesion area and reendothelialisation of the vascular intima were observed by Ivans'blue staining,and the intima hyperplasia and lumen stenosis were observed by hematoxylin-eosin(HE)staining.Result In the sham operation group,the vascular intima was smooth,the endothelium was intact,and there was no hyperplasia in the vascular intima.After 14 days of balloon injury,endothelial hyperplasia was obvious in control group,and the area of neointimal was significantly higher than that in sham operation group,the difference was statistically significant(P<0.05).After 14 days of balloon injury,the area of neointimal in the experimental group was significantly lower than that in the control group,and the proportion of reendothelialization area in the injured arteries was significantly higher than that in the control group,with statistical significance(P<0.05).Conclusion The effects by which nicorandil can facilitate reendothelialization and attenuate intimal hyperplasia of animal model of balloon injury in rat carotid artery are marked.
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万方数据
维普
