Effects of Three Nesting Materials and Placement Levels on the Nesting and Breeding Performance of Mice
Objective To investigate the effects of three nesting materials and placement levels on the nesting and breeding performance of mice.Method C57BL/6JGpt(B6)mice and BALB/cNj-Foxn1nu/Gpt(BALB/c-nu)mice were randomly divided into 12 groups(n=36,trio-bred).Mice were provided with three kinds of nesting materials(raffia,paper cotton and paper tissue)and four placements(2 g,4 g,6 g and 8 g)levels respectively.Nesting scores were assigned on the first week after mating,and total litter size,total weaning pups and weaning weight of pups were counted weekly for 5 months.Result(1)The nesting scores of the raffia and paper tissue groups were significantly better than those of the paper cotton group in both strains(P<0.05),and there was no significant correlation between nesting scores and the placement level of nesting material(P>0.05).(2)There was no significant correlation between total litter size,total weaning pups and the kinds of nesting material in both strains(P>0.05);the total litter size and total weaning pups in the 6 g paper tissue group of B6 strain was significantly better than that in the 2 g paper tissue group(P<0.05);the total litter size in the 2 g paper tissue group was significantly better than that in the 6 g paper tissue group(P<0.05),and total weaning pups in the 2 g nesting material groups were significantly better than that in the 8 g nesting material groups(P<0.05);there was no significant correlation between weaning weight of pups and types and placement levels of nesting material in both two strains.Conclusion There were differences between the two strains for types and placement levels of nesting materials.