Effect of Three Animal Anesthetics on Anesthetic Efficacy and Serum Biochemical Indicators in C57BL/6J Mice
Objective To compare the anesthetic effects of three animal anesthetics:tribromoethanol,pentobarbital Sodium,zoletil 50 combined with serazine hydrochloride and their effects on serum biochemical indicators in mice.To explore the suitable dose range of three anesthetics for anesthesia of B6 mice.Method 100 healthy SPF grade male B6 mice were randomly divided into 10 groups,with 10 mice in each group.Three types of anesthetics were administered intraperitoneally to B6 mice at high,medium,and low dose gradients,respectively.Compared the induction time,maintenance time,recovery time,and effects on serum ALT、AST、GLU and UA indicators of B6 mice under different doses of three anesthetics.Result The anesthesia maintenance time and recovery time of the three anesthetics were proportional to the dose,while the induction time was inversely proportional to the dose.Medium(250 mg/kg)low(125 mg/kg)doses of tribromoethanol(1.25%),medium(80 mg/kg)low(40 mg/kg)doses of pentobarbital sodium(1.0%),and medium(60 mg/kg)low(30 mg/kg)doses of Zoletil 50(0.50%)combined with serazine hydrochloride(0.50%,medium dose 12 mg/kg,low dose 6 mg/kg)had moderate anesthesia related time and little impact on liver and kidney function in B6 mice.Conclusion The anesthetic effects and serum biochemical indicators of the three anesthetics on B6 mice were different.Tribromoethanol had a suitable anesthesia effect in the range of 125-250 mg/kg;Pentobarbital sodium had a suitable anesthetic effect in the range of 40-80 mg/kg;Zoletil 50 combined with serazine hydrochloride had a suitable anesthesia effect within the range of 30-60 mg/kg(Serazine Hydrochloride 6-12 mg/kg).In studies involving ALT,AST,GLU,and UA,appropriate doses of anesthetics need to be selected based on different experimental needs.
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NSTL
万方数据
维普
