Effects of Different Fixing Solution on Hippocampal Neurons of Golden Hamster under Light Microscopee
Objective To compare the effects of different fixations on neurons in hippocampus of golden hamster under light microscope,and to provide reference for the selection of fixations in hippocampus of golden hamster.Method 21 11-week-old male SPF golden hamsters were randomly divided into 7 groups.The heart was injected with 4%paraformaldehyde,10%neutral formaldehyde,Bouin's,Carnoy,Davidson's,Zenker and Helly solution,and the whole brain was removed.It was fixed in the corresponding fixing solution for 48 h,and made into paraffin sections.After HE staining,the morphological characteristics of hippocampus neurons were observed under the light microscope.The diameter of neuronal cells was measured by the measuring scale of 3DHISTECH digital slice scanning system in Hungary,and the effect of fixation solution on cell contraction was compared.Result 4%paraformaldehyde,10%neutral formaldehyde,Bouin's solution showed a good fixing effect,the fixed brain hardness is moderate,HE stained slices with moderate coloring,bright color,neuron cell morphology contrast is clear,4%paraformaldehyde is more ideal because the slices without cracking after fixation.Hippocampal neurons immobilized with different fixations showed significant differences in cell shrinkage(P<0.01),and the cell shrinkage of neurons immobilized with Carnoy and Davidson's was the most severe.Conclusion 4%paraformaldehyde,10%neutral formaldehyde and Bouin's solution are ideal fixation fluids for hippocampus neurons of golden hammers,and 4%paraformaldehyde solution is recommended as the first choice.
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