Experimental Study on the Inhibitory Effect of Sodium Danshensu on Ototoxicity of Cisplatin
Objective To investigate the inhibitory effect of Sodium Danshensu(SDSS)on ototoxicity of cisplatin.Method HEI-OC1 cell lines were selected to construct cisplatin-induced damage models based on which protective agents were screened in the small-molecule compound library and Determination of IC50 of cisplatin in HEI-OC1 cells at 24 h.The effect of SDSS on hair cell survival was measured in HEI-OC1 cell models and cisplatin-induced mouse cochlear implant models.The effect of SDSS on cisplatin-induced oxidative stress levels in HEI-OC1 cells was measured by superoxide anion fluorescence probe(DHE)staining and the positive control was NAC.Result SDSS,a potent protective agent,was selected from 210 FDA-approved small molecule compounds,and SDSS pretreatment significantly inhibited cisplatin-induced decrease in HEI-OC1 cell activity,apoptosis and reactive oxygen species(ROS)aggregation at an optimal concentration of 200 μmol/L.In addition,SDSS significantly inhibits cisplatin-induced damage to hair cells in mouse cochlear explants.Conclusion SDSS may play a protective role in cisplatin-induced ototoxicity by inhibiting ROS aggregation and apoptosis in hair cells.
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