猴椅保定训练对恒河猴应激的影响
Stress Effect of Monkey Chair Restraint Training in Rhesus Macaques
赵琪 1刘永涛 2周超 2兰艳丽 2汪溪洁 1李彩云2
作者信息
- 1. 上海益诺思生物技术股份有限公司,上海 201203;中国医药工业研究总院,上海 201203
- 2. 上海益诺思生物技术股份有限公司,上海 201203
- 折叠
摘要
目的 考察恒河猴猴椅保定 30 min连续 5 次适应性对应激激素水平的影响.方法 约 3 岁恒河猴,雌雄各15 只,分 3 组,每组 10 只,雌雄各半进行猴椅保定适应性训练,每间隔 1d训练 1 次,每次训练 30 min.组 1:不进行适应性训练组,组 2:单纯适应性训练组,组 3:给予零食和安抚训练组.共训练 5 次,每次训练结束对 3 组所有动物采血分析.结果 与组 1 相比,组 2 和组 3 在多次猴椅保定适应性训练后可见猴血清皮质酮(CROT)显著性降低(P<0.05 或 P<0.01),组 3 的猴 β-内啡肽(β-EP)显著降低(P<0.05 或 P<0.01),组 3 于第 1 和第 5 次适应性训练后 5-羟色胺(5-HT)升高(P<0.01).结论 进行猴椅保定的适应性训练能显著降低恒河猴的应激压力,让动物趋于习惯或适应坐猴椅保定的操作.在适应性训练中,给予正向强化的训练方式让受试者在减少应激和攻击性表现更显著,适应性保定训练 3～4 次就可以让应激压力降到恒定水平.
Abstract
Objective To investigate the effects of five times in half an hour on stress hormone levels in Rhesus Macaques.Method 3 years old Rhesus Macaques,15 males and 15 females were divided into three groups,5 males and 5 females in each group were trained in monkey chair restraint once every two days for 30 min each time.Group 1:no restraint training group,group 2:only restraint training group,group 3:give treats and comfort training group.A total of 5 training times,all the animals in the three groups were collected blood for analysis after each training.Result Compared with group 1,CORT was significantly decreased in groups 2 and 3 after multiple adaptive training of monkey chair preservation(P<0.05 or P<0.01),β-endorphin in group 3 was significantly decreased(P<0.05 or P<0.01),and 5-HT was increased in group 3 at the first and fifth adaptive training(P<0.01).Conclusion When a restraint with monkey chair used in Rhesus Macaques last for half an hour,the adaptive training for the monkey chair restraint can significantly reduce stress.The animals tend to get used to or adapt to the operation of the monkey chair restraint.In adaptive training,positive reinforcement training was given to reduce stress and aggression significantly,and 3-4 times of adaptive training in the monkey chair reduced stress to a constant level.
关键词
非人灵长类/保定/应激/皮质酮/内啡肽/5-羟色胺Key words
non-human primates/restraint/stress/CORT/endorphin/5-HT引用本文复制引用
出版年
2024
NETL
NSTL
维普
万方数据