对氯苯丙氨酸建立失眠模型行为学评价方法的研究
Study on Behavioral Evaluation Method for Establishing Insomnia Model with Para-chlorophenylalanine
曲子涵 1马海钊 1张野 1刘英豪 1郅晓宇 1李宝杰 1张红石1
作者信息
摘要
目的 通过观察对氯苯丙氨酸(PCPA)失眠大鼠模型在造模成功后一周内,各类行为学实验中的学习记忆能力改变,验证 PCPA失眠模型稳定性.方法 30 只 SD大鼠随机均分为空白组和模型组,空白组正常饲养,对模型组大鼠腹腔注射 PCPA建立失眠模型,建模后第 1 周进行 Morris水迷宫实验等 5 项大鼠行为学监测.结果 与空白组比较,模型组大鼠睡眠潜伏期显著延长(P<0.01),睡眠持续时间显著缩短(P<0.01);在水迷宫实验中,模型组大鼠寻台时间延长(P<0.01);巴恩斯迷宫实验中,模型组大鼠测试探索期的目标洞口停留总时间显著缩短(P<0.01);避暗实验中,模型组错误次数增多(P<0.05),且潜伏期显著延长(P<0.01);高架十字迷宫实验中,模型组大鼠开臂停留时间显著缩短(P<0.01);旷场实验显示模型组大鼠在中央区域的停留时间、直立次数和运动总距离均低于空白组,其中中央区域的停留时间具有显著差异(P<0.01).结论 PCPA失眠模型具有较高的可重复性和稳定性,可以作为睡眠和记忆相关研究的基础模型.
Abstract
Objective By observing the changes in learning and memory abilities in various behavioral experiments of the para-chlorophenylalanine(PCPA)insomnia rat model within a week after successful modeling,the stability of the PCPA insomnia model was verified.Method Thirty SD rats were randomly divided into blank group and model group,the rats in the blank group were fed normally,and establishment of insomnia model by intraperitoneal injection of PCPA into model group rats.Five behavioral tests including Morris water maze test were performed in the first week after modeling.Result Compared with the blank group,the sleep latency of the model group was significantly prolonged(P<0.01),and the sleep duration was significantly shortened(P<0.01).In the water maze experiment,the time of seeking platform in the model group was prolonged(P<0.01).In the Barnes maze experiment,the total residence time at the target portal of the model group rats during the test exploration period was significantly shortened(P<0.01).In the dark avoidance experiment,the number of errors in the model group increased(P<0.05),and the latency was significantly prolonged(P<0.01).In the elevated cross maze experiment,the open arm residence time of rats in the model group was significantly shortened(P<0.01).In the open-field experiment,the dwell time,the number of uprightness and the total distance of movement in the central area of the model group were all lower than those of the blank group,among which the dwell time in the central area was significantly different(P<0.01).Conclusion The PCPA insomnia model has high reproducibility and stability,and can be used as a basic model for sleep and memory-related studies.
关键词
PCPA/失眠模型/行为学评价Key words
PCPA/insomnia model/behavioural evaluation引用本文复制引用
基金项目
国家自然科学基金项目-面上项目(82074569)
2021年大学生创新创业训练计划项目(S202110199081)
出版年
2024
NETL
NSTL
万方数据