Exploration of the Effect of Specific Immunity on Inflammatory Response in Asthmatic Mice Bronchoalveolar Lavage Fluid Based on Th1/Th2 Expression
Objective To explore the effect of specific immunity on inflammation in bronchoalveolar lavage fluid(BALF)of asthmatic mice by analyzing Th1/Th2 expression.Method Forty mice were divided into control group,model group,SIT group,and dexamethasone group,with 10 mice in each group.Except for the control group,asthma mouse models were established in the other groups.After the stimulation phase,the SIT group was injected with 1 mg of OVA,the dexamethasone group was given a 0.075 mg/mL solution,and the remaining rats were injected with an equal volume of PBS solution.The lung function meter was used to detect airway responsiveness;the BALF inflammatory cells were observed by Wright-Giemsa staining;ELISA was used to detect cytokines IL-13,IL-4,IL-5,and INF-γ in BALF;HE staining was used to observe lung tissue morphology;PAS staining was used to observe cup-shaped cell activity in lung airways;and flow cytometry was used to detect the proportion of Th1 and Th2 in lung tissues.Result Specific immunity significantly reduced airway hyperreactivity in model mice,reduced inflammatory cell activity in BALF,and inhibited asthma occurrence and development by increasing Th1 while decreasing Th2.Conclusion Specific immunity can alleviate airway hyperresponsiveness and inhibit inflammation in asthmatic mice,improve cup-shaped cell proliferation,and relieve ventilation,possibly related to regulating Th1/Th2 expression.
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