Effect of Different Dehydration and Wax Immersion Procedures on the Quality of Paraffin Sections of Mouse Liver and Intestine
Objective To compare the effects of two dehydration procedures and five wax immersion times on the quality of paraffin sections of mouse liver and intestinal tissues,with a view to optimizing the preparation procedures of paraffin sections.Method The liver and intestinal tissues of BALB/c mice were prepared in two different dehydration procedures(Procedure Ⅰ and Procedure Ⅱ)and five different wax immersion procedures(total immersion time of 90,120,150,180 and 210 min)to compare the result of tissue sectioning and HE staining.Result The paraffin sections produced by dehydration procedure 1 were easily fragmented,with obvious wrinkles and cavities in the center of the tissue;the paraffin sections produced by dehydration procedure 2 were intact and free of cavities,and the structure of the paraffin sections was observed to be intact and clear under the microscope after HE staining.Compared with the other four wax immersion procedures,the paraffin sections produced with a total immersion time of 150 min were the most complete and clear after staining.Conclusion The quality of the paraffin sections produced by using the dehydration procedure Ⅱ and the total time of wax immersion of 150 min was improved and easy to observe under the microscope,which is recommended as the optimized procedure for paraffin sections of mouse liver and intestinal tissues.
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