Development of Reproductive System of Mongolian Gerbil Before and After Sexual Maturity
Objective To investigate in depth the development of the reproductive system during sexual maturation in long-clawed gerbils,and to provide direct research evidence for the exact time of sexual maturation.Method A total of 150 long-clawed gerbils were selected from 6 to 14 weeks of age and divided into 5 groups of 15 males and 15 females according to the ages of 6,8,10,12 and 14 weeks,weighed and collected samples of ovaries,uterus,testes and epididymis,measured the wet weight of the samples and calculated the index of the organisms;the morphology of the ovaries was observed under the microscope,and the spermatozoa were detected by a cytometer;6 samples were randomly selected from each group for HE staining to observe the development of the ovaries,testes and epididymis.Result In female gerbils,ovarian wet weight and ovarian index increased significantly at 10 weeks of age,and there was no significant change between 10 and 14 weeks of age;uterine wet weight and uterine index changed in stages;under microscopic observation,the incidence of corpus luteum in the groups of 10 weeks of age,12 weeks of age and 14 weeks of age was 46.7%,60%and 73.3%,respectively;the tissue sections showed that corpus luteum could be seen clearly from 10 weeks of age.In male gerbils,the wet weight of testes increased gradually with the increase of age from 6 weeks to 10 weeks,but there was no significant change after 10 weeks;the testicular index increased significantly at 8 weeks and 12 weeks of age,and decreased significantly at 14 weeks of age;both epididymal wet weight and epididymal index increased gradually with weekly age from 6 weeks of age to 12 weeks of age,and there was no significant change in epididymal wet weight at 14 weeks of age,and the epididymal index decreased significantly.The result of the cell counter showed that the sperm count was zero at 6 weeks of age,the sperm count in the epididymis increased significantly at 10 weeks of age compared to 8 weeks of age,and there was no significant change between 10 and 14 weeks of age.Tissue sections showed that a small number of spermatozoa began to appear in the testis and epididymis from 8 weeks of age and a large number of spermatozoa were visible from 10 weeks of age.Conclusion As the gerbil's age increases,the ovaries of female gerbils begin to develop and discharge follicles and eggs at about 10 weeks of age,and the gerbil reaches sexual maturity and the ability to reproduce.Male gerbils began to produce sperm at around 8 weeks of age and entered the first estrus.At around 10 weeks of age,the testes developed stably and the spermatozoa reached the maximum number,reaching sexual maturity,after which the epididymis continued to develop,and its development stabilised at around 12 weeks of age.
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