Changes of Some Serum Inflammatory Cytokines in Type 2 Diabetes Model of Minipigs
Objective To dynamically observe the levels of inflammatory cytokines in the serum of type 2 diabetes(T2DM)model in miniature pigs,so as to provide reference data for the study of type 2 diabetes.Method Ten Bama minipigs were randomly divided into a Control group and a model group(DM).The Control group was fed with conventional diet,and the DM model in miniature pigs was established by high-fat diet combinated with intravenous streptozotocin(STZ).The experiment lasted for nine months,fasting blood glucose and insulin were measured monthly,and glucose tolerance was measured every 3 months.Concentrations of 11 inflammatory cytokines in serum of animals at the 0th,3rd,6th,and 9th months were determined by ELISA kit.Result The fasting blood glucose was significantly increased(P<0.05),severely impaired of glucose tolerance and insulin resistance occurred in the DM group after high-fat diet for 3 months injected with STZ.The ELISA result showed that there was no significant change in the levels of 11 inflammatory cytokines of the DM group at the first 3 months,and no significant difference compared to the Control group.The levels of platelet-derived growth factor-BB(PDGF-BB)and tumor necrosis factor-α(TNF-α)were increased after the onset of T2DM and significantly higher than those of in Control group(PDGF-BB,P<0.05 at the 6th month;PDGF-BB and TNF-α,P<0.05 at the 9th month).The level of interleukin-6(IL-6)in the DM group was increased gradually over time which the level at the 9th month was significantly higher than that at the 0th and 3rd month(P<0.05),but no significant difference compared with the Control group.Conclusion The model of type 2 diabetes in minipigs was successfully established by using high-fat diet combined with low-dose STZ.A simply high-fat diet was not obvious influence on the levels of inflammatory cytokines in serum of mini-pigs,whereas the levels of PDGF-BB and TNF-α in serum of T2DM mini-pigs were significantly increased and higher than those in normal animals.
type 2 diabetes mellitusinflammatory cytokineshigh-fat dietSTZmini-pig
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