"一"字型高架迷宫在小鼠行为学测试中的应用
Application of"—"Maze in Behavioral Testing of Mice
高明周 1胡明会 2周婉晴 2刘梦迪 2韩穆轩 2耿希文 2魏盛 2张浩2
作者信息
- 1. 山东中医药大学创新研究院,济南 250355
- 2. 山东中医药大学实验中心,济南 250355
- 折叠
摘要
目的 验证"一字型"高架迷宫("—"Maze)测试动物焦虑状态的客观性和准确性,主动激发大鼠、小鼠探索行为,有效解决焦虑三联测试装置(旷场+高架十字迷宫+明暗箱)中高架十字迷宫(EPM)的测试缺陷.方法 ①进行"—"Maze行为学测试,并与 EPM 进行对比;②通过旷场实验和明暗箱实验,检测"—"Maze 对动物有无其他行为影响;③通过药物干预(地西泮,Diazepam,DZP,焦虑症治疗药物),验证"—"Maze 的测试效果.结果 与EPM 比较,"—"Maze开放臂进入时间和次数显著增加(P<0.05,P<0.001);与 EPM 组相比,EPM 组+地西泮组(EPM+DZP)开放臂进入时间和次数显著增加(P<0.05,P<0.01);与 EPM 组相比,EPM+DZP 组旷场中央区运动路程和进入次数显著增加(P<0.05).与"—"Maze 组相比,"—"Maze+地西泮("—"Maze+DZP)组旷场中央区运动路程、进入时间和次数显著增加(P<0.05).与 EPM 组相比,EPM+DZP 组明暗箱明区进入时间和次数显著增加(P<0.05,P<0.01).与"—"Maze 组相比,"—"Maze+DZP 组明暗箱明区进入时间和次数显著增加(P<0.05).结论 "—"Maze开放臂进入时间和次数显著优于 EPM,且不会对动物的行为产生其他影响,更加适合于焦虑模型测试,值得进一步研发和推广应用.
Abstract
Objective Verify the objectivity and accuracy of the"—"Maze in testing animal anxiety states,actively stimulate exploratory behavior in rats and mice,and effectively solve the testing defects of the elevated plus maze(EPM)in the anxiety triple testing device(open field+elevated plus maze+light-dark box).Method ①Conduct a"—"elevated maze test and compare it with EPM;②Through open field test and light-dark boxtest,detect whether the"—"elevated maze has other behavioral effects on animals;③Verify the testing effect of"—"Maze through drug interventions(such as Diazepam,DZP,the anxiety therapy drugs).Result Compared with EPM,the"—"Maze group showed a significant increase in open arm entry time and frequency(P<0.05,P<0.001).Compared with the EPM group,the EPM+Diazepam group(EPM+DZP)showed a significant increase in open arm entry time and frequency(P<0.05,P<0.01).Compared with the EPM group,the EPM+DZP group showed a significant increase in the distance and number in the central area of the open field test(P<0.05).Compared with the"—"Maze group,the"—"Maze+Diazepam group("—"Maze+DZP)showed a significant increase of the distance,entry time,and frequency in the central area of the open field test(P<0.05).Compared with the EPM group,the EPM+DZP group showed a significant increase in the time and frequency of light-dark box test(P<0.05,P<0.01).Compared with the"—"Maze group,the"—"Maze+DZP group showed a significant increase in the time and frequency of light-dark box test(P<0.05).Conclusion The open arm entry time and frequency of the"—"Maze are significantly better than those of EPM,and do not have any other effects on animal behavior.It is more suitable for anxiety model testing and is worth further research and development and promotion.
关键词
高架十字迷宫/一字型高架迷宫/行为学/焦虑Key words
elevated plus maze/"—"elevated maze/behavioral studies/anxious引用本文复制引用
出版年
2024
NETL
NSTL
万方数据