Renal Injury in Streptozotocin-induced Diabetic Rats
Objective To establish a diabetic rat model induced by streptozotocin and evaluate its renal injury.Method Four-week-old male Sprague-Dawley(SD)rats were fed a high-fat diet and injected intraperitoneally with streptozotocin(STZ)at a dose of 30 mg/kg to induce the diabetic model.During the experiment,the general condition of the rats was observed,and fasting blood glucose(FBG)and body mass was measured.A FBG level≥11.1 mmol/L was used as the standard for establishing the type 2 diabetes model.After successful modeling,the following parameters were assessed:24-hour urine protein(UP),urine creatinine(UCr),serum creatinine(SCr),and blood urea nitrogen(BUN)content.Renal tissue was collected to calculate the kidney index(KI),and HE staining,periodic acid-Schiff(PAS)staining,and Masson staining were performed to observe renal injury.Result Compared with the normal group,the model group showed decreased body weight and urine creatinine levels,while 24-hour urine protein,serum creatinine,BUN content,and kidney index were significantly increased(all P<0.05).No obvious abnormalities were observed in the renal pathological staining.Conclusion SD rat model of type 2 diabetes can be established through a 13-week high-fat diet and STZ injection(30 mg/kg).This model causes renal injury;however,no pathological changes such as glomerulosclerosis,mesangial cell proliferation,or renal fibrosis were observed under light microscopy.
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