Dynamic Changes and Differentiation Trends of Blood Lipid Levels with Age in Long Clawed Gerbils
Objective Exploring the changes and differentiation trends of blood lipids in long clawed gerbils of different ages.Method Select 20 long clawed gerbils each aged 2,4,6,8,10,12,14,and 16 months and feed them with regular rat feed,with half male and half female.After euthanasia of animals,serum was obtained for the detection of four indicators:total cholesterol(TC),triglycerides(TG),high-density lipoprotein(HDL-C),and low-density lipoprotein(LDL-C).The blood lipid levels of long clawed gerbils at different stages of natural growth and development were compared,and they naturally differentiated into normal blood lipid groups and abnormal blood lipid groups.At the same time,two samples of the largest lobe of the liver were collected,one for histological observation using routine HE staining,and the other for observation of collagen fiber accumulation using Sirius red staining.Result There were no abnormalities in blood lipids in the 2-month-old and 4-month-old groups,while serum triglycerides(TG)and total cholesterol(TC)indicators began to increase in 6-month-old gerbils.From 8-month-old,serum TC levels in animals with dyslipidemia compared to those with normal blood lipids began to increase TG was significantly elevated(P<0.01),and serum LDL-C was significantly increased in 8-month-old and 16-month-old animals with dyslipidemia compared to animals without dyslipidemia(P<0.01).Among animals with abnormal blood lipids,the 8-month-old group accounts for only 20%,while the proportions of 10,12,and 14-month-old do not exceed 50%.The proportion of animals in the 16-month-old group has reached 70%,and there are more male animals.Pathological examination showed that the liver metabolism of the 2-month-old group of animals was vigorous,with binuclear liver cell division and no other abnormalities found.Fat vacuoles began to appear in the 8-month-old animals,and a large number of liver cells died in the 16-month-old group.The liver cord was unclear,and the hepatic sinusoids were narrowed or even disappeared.Severe steatosis occurred in liver cells,with mild increase in collagen fibers at 14 months old and further significant accumulation of collagen fibers at 16 months old.Conclusion Feeding male long clawed gerbils with regular rat feed up to 16 months old can exhibit typical and stable lipid abnormalities,providing basic support for the study of replicating self occurring hyperlipidemia animal models with similar lesions to humans.
long clawed gerbilsabnormal blood lipidsage changes
万方数据
维普
