High-fat Diet was Used to Establish the Model of Blood Hypercoagulability in Mice
陈文轩 1罗小英 2全志浩 2胡学瀚 2胡晶3
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作者信息
1. 重庆医科大学第二临床学院,重庆 400016
2. 重庆医科大学第一临床学院,重庆 400016
3. 重庆医科大学检验医学院,临床检验诊断学教育部重点实验室,重庆 400016
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摘要
目的 探究高脂饲料喂养 KM 小鼠造成小鼠血液高凝状态的可行性,为高凝状态的研究提供合适、简便的动物造模方法.方法 将 KM 小鼠随机分为对照组和实验组两组:对照组用普通饲料喂养,实验组用高脂饲料(脂肪供能占比 60.65%)喂养.于第 30 天测量小鼠体质量并采尾静脉血测定小鼠活化部分凝血酶原时间(activated partial thromboplastin time,APTT)、凝血酶原时间(prothrombin time,PT),通过比较对照组与实验组小鼠血液凝血指标的差异,判断高脂饲料喂养是否能够造成小鼠血液高凝状态的发生.结果 在相同的饲养条件及采血止血方式下,相较于对照组,实验组通过高脂饲料喂养 30 d 后,KM 小鼠体质量明显升高(P<0.001),PT、APTT 明显缩短且有统计学意义(均 P<0.01),实验组小鼠血液呈现高凝状态.结论 通过高脂饲料喂养可成功构建 PT、APTT 缩短的小鼠高凝状态模型.
Abstract
Objective To explore the feasibility of high-fat diet feeding KM mice to cause hypercoagulable state of blood in mice,and to provide a suitable and simple animal modeling method for the study of hypercoagulable state.Method KM mice were randomly divided into two groups:control group and experimental group,Control group was fed normal diet(maintenance diet)and the experimental group was fed high fat diet(fat for energy 60.65%).On the 30th day,the body weight of the mice was measured and the tail venous blood was collected to determine the activated partial thromboplastin time(APTT)and prothrombin time(PT)of the mice.By comparing the differences in blood coagulation indexes between the control group and the experimental group,it was determined whether high-fat diet feeding could cause the occurrence of hypercoagulable state in mice.Result Under the same feeding conditions and blood collection and hemostasis method,compared with control group,mice in the experimental group were fed a high-fat diet for 30 days,the body weight of mice in high-fat diet group was significantly incread(P<0.001),and PT and APTT were significantly decreased(P<0.01),with statistical significance,and the blood of mice in high-fat diet group showed hypercoagulability.Conclusion The hypercoagulable state model of mice with shortened PT and APTT can be successfully constructed by high-fat diet.
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