Analysis of Biological Mechanism of Postpartum Rat Model Based on RNA-Seq
Objective To analyze the differential expression of genes in postpartum rat animal model by RNA-Seq and characteristics of biological mechanism.Method 9 10-week-old female SD rats were divided into Y0,Y1 and Y2 group randomly,with 3 in each group.RNA was extracted from the abdominal aorta blood of postpartum rats,and transcriptome sequencing was performed using RNA Seq technology.The differentially screened genes were subjected to GO and KEGG bioinformatics analysis.Result Compared Y0,there were 1 222 significantly different genes in both Y1 and Y2.GO analysis showed that significantly different genes were mainly expressed in biological processes related to immunity,oxidative stress and metabolism.KEGG analysis showed that different genes were mainly related to the signal pathways of immune system,signal transduction,signal molecules and interactions,cell growth and death,transportation and catabolism.Conclusion Postpartum rat could simulate the changes of biological function of human postpartum condition as an animal model,could used to observe the impact of pregnancy and childbirth on biological function of the body and explore effective intervention measures.
pregnancy and childbirthanimal modelRNA-sequencing
万方数据
维普
