Characteristics of Gut Microbiota in Representative Rat Strains via 16S rDNA Sequencing Technology
Objective 16S rDNA sequencing was used to study on the characteristics of gut microbiota in four representative rat strains.Method The samples of fresh feces were collected from 48 SD,Wistar,F344,and BN rat strains were collected from the same feeding facility,with half male and half female.The V4 region was amplified with 16S rDNA universal primers.The composition and diversity was analyzed with QIIME.The LEfSe software was used to compare major flora with statistical differences in each strains and gender.Result Bacteroidetes and Firmicutes constitute the main intestinal flora of the rats.Muribaculaceae,Bacteroidesare the main intestinal flora on genera level.The Alpha diversity showed that compared with inbred rats,the outbred rats had higher diversity and richness with higher dispersion.Beta diversity showed that the structure of the gut microbiota from different strains and different genders are different.Conclusion The characteristics of gut microbiota of representative rat strains from National Rodent Laboratory Animal Resources Center is analyzed to rich date support for the related research,which also showed that main intestinal flora from the same breeding condition is similar,and the diversity and richness of the gut microbiotais related to strain and gender.
万方数据
维普
