Synergistic denitrification effect of hydroxylamine enhanced sulfur autotrophic denitrification and anaerobic ammonia oxidation
In view of the inhibitory effect of sulfide substrate on the performance of the coupling autotrophic sulfur denitrifica-tion and anammox system,hydroxylamine was added gradually into the system to achieve efficient nitrogen removal using UASB reactor.The results show that the total nitrogen removal load reaches as high as 1.05 kg/(m3·d)at HRT of 4 h with the influent NH+4-N and NO-3-N concentration of 60 mg/L and 125 mg/L,respectively.The contribution of anammox for TN removal is among 76%~95%,which is 1.55 times that without NH2 OH.The addition of hydroxylamine promotes heme c pro-duction and EPS secretion,and the ratio of NH2 OH to S2-higher than 0.024 is helpful to the enhancement of SAA.The a-bundance and activity of Candidatus_Kuenenia is significantly increased in the presence of hydroxylamine,so as to achieve efficient nitrogen removal by cooperating with sulfur autotrophic denitrification bacteria Thiobacillus,Thiothrix,Sulfuri-monas,Sulfurovum and Sulfuritalea.
hydroxylammoniaautotrophic sulfur denitrificationanammoxnitrogen removalmicrobial community