PDLIM3在子宫内膜异位组织中的表达及临床意义
The Expression of PDLIM3 in Endometriosis and its Clinical Significance
徐亦聪 1廉鑫 2杨雨青 1周圣涛1
作者信息
- 1. 四川大学华西第二医院妇产科,四川 成都 610041
- 2. 南京大学医学院附属鼓楼医院妇产科,江苏 南京 210093
- 折叠
摘要
目的:研究细胞骨架蛋白PDZ和LIM结构域蛋白3(PDLIM3)在子宫内膜异位症(EMT)患者的异位内膜病灶、在位子宫内膜及正常子宫内膜中的表达水平差异及临床意义.方法:通过基因表达综合数据库(GEO)中的多个独立数据集对PDLIM3 mRNA的表达水平进行生物信息学分析;选取四川大学华西第二医院2019 年12 月至2020 年7 月收治的58 例EMT患者和27 例其他妇科良性疾病患者,通过EMT患者获取异位病灶组和在位内膜组样本,通过其他妇科良性疾病患者获取正常内膜对照组样本.采用实时荧光定量核酸扩增法、免疫印迹法和免疫组织化学法检测不同组织样本中的PDLIM3 表达水平,并结合相关临床参数探究其临床意义.结果:相比患者在位内膜和对照组正常内膜组织,PDLIM3 mRNA(F =46.05,P<0.05)及蛋白(F =238.60,P<0.05)在异位子宫内膜病灶中的表达水平显著上调;通过免疫组化实验获得的异位子宫内膜病灶中PDLIM3 平均光密度值与EMT患者的痛经评分(r =0.86,P<0.05)及血CA 125 水平(r =0.41,P<0.05)呈正相关,但与EMT患者的疾病分期、经量多少和CA 199水平无明显相关(P>0.05).结论:PDLIM3 在异位内膜病灶中的表达水平显著上调,且其表达水平与EMT患者的痛经程度和CA 125 水平相关,提示PDLIM3在EMT的发生发展过程中可能发挥重要作用,并可能作为治疗EMT的潜在靶点.
Abstract
Objective:To investigate the expression of PDLIM3 in the ectopic and orthotopic endometrium of endometriosis(EMT)patients,as well as in healthy endometrium,and to explore its relationship with clinical signifi-cance.Methods:Bioinformatics analysis based on the Gene Expression Omnibus(GEO)database was conduc-ted,and a prospective cohort study was undertaken,involving 58 EMT patients treated at West China Second Hospital of Sichuan University and 27 patients with other benign gynecological diseases as the control group from December 2019 to July 2020.Ectopic and orthotopic endometrium samples were obtained from EMT patients,and normal control samples were obtained from patients with other benign gynecological diseases.Real-time quantita-tive polymerase chain reaction(qPCR),western blot,and immunohistochemistry were utilized to investigate PD-LIM3 expression in various tissue samples.Clinical parameters were analyzed to determine the correlation be-tween PDLIM3 and clinical features.Results:Compared to orthotopic endometrial tissues of EMT patients and normal endometrial tissues of control subjects,the mRNA(F =46.05,P<0.05)and protein(F =238.6,P<0.05)expression levels of PDLIM3 in ectopic endometrial lesions were significantly upregulated.The average optical density(AOD)of PDLIM3 protein obtained through immunohistochemical experiments in ectopic endometrial le-sions showed a positive correlation with the degree of dysmenorrhea(r =0.86,P<0.05)and CA 125 level(r = 0.41,P<0.05)in EMT patients.But no obvious correlation was found between the expression level of PDLIM3 and disease stage,menstrual volume,and CA199 level(P>0.05).Conclusions:The expression level of PDLIM3 in endometriosis lesions was significantly upregulated and correlated with the degree of dysmenorrhea and serum CA125 level.These findings suggest that PDLIM3 may play a significant role in the development and progression of endometriosis and could serve as a potential therapeutic target.
关键词
子宫内膜异位症/PDZ和LIM结构域蛋白3/痛经Key words
Endometriosis/PDLIM3/Dysmenorrhea引用本文复制引用
出版年
2023
国家科技期刊平台
NSTL
万方数据