The application value of human papillomavirus genotyping detection in HPV infection in postmenopausal women
Objective To explore the application value of human papillomavirus(HPV)genotyping in postmenopausal women with HPV infection.Methods 80 postmenopausal women who underwent HPV screening were selected as the study subjects,and their cervical exfoliated cell samples were collected and detected by polymerase chain reaction(PCR)-reverse hybridization.The DNA was extracted by column extraction,and the extracted solution,negative and positive control products were amplified by Tianlong PCR and hybridized by Ambipine hybridization.The positive rate,risk typing,typing of positive and infected samples,and positive rate of HPV infection in women of different ages were analyzed.Results Among 80 postmenopausal women,the HPV positive rate was 15.00％(12/80).Among them,HPV-16 type 2 cases,accounting for 16.67％;HPV-52 type 2 cases,accounting for 16.67％;HPV-58 type 1 case,accounting for 8.33％;HPV-53 type 1 case,accounting for 8.33％;HPV-68 type 1 case,accounting for 8.33％;HPV-33 type 1 case,accounting for 8.33％;HPV-11 type 1 case,accounting for 8.33％;HPV-18 type 1 case,accounting for 8.33％;HPV-cp8304 was found in 1 case,accounting for 8.33％.HPV-66 type 1 case,accounting for 8.33％.Among the 12 HPV-positive samples,3 were multiple infected,accounting for 25.00％;9 were single infected,accounting for 75.00％.There was no significant difference in the distribution of HPV positive cases in different age groups(P＞0.05).Conclusion There was no obvious distribution of HPV positive rate in postmenopausal women of different ages,and the infection was mainly single infection.HPV genotyping has a high diagnostic value and is worth promoting.
Human papillomavirusVirus genotypingPostmenopausal woman
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NSTL
万方数据
