首页|电化学法制备碳点荧光探针测定氯霉素含量的研究

电化学法制备碳点荧光探针测定氯霉素含量的研究

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以葡萄糖作为碳源,通过电化学碳化的方法制备碳点(C-dots)并表征其形貌和光学性质.将C-dots作为荧光探针检测氯霉素的含量.研究表明,所制备的C-dots在紫外光的激发下发射蓝色荧光(激发/发射波长分别为 345/450 nm),氯霉素对C-dots荧光有猝灭作用,且C-dots荧光猝灭效率与氯霉素浓度呈现良好的线性关系,线性范围为 0.1~5.0 mmol/L,检测下限为0.016 mmol/L(信噪比S/N=3).该方法制备的C-dots具有优异的荧光性能及光学稳定性,可灵敏、便捷地检测氯霉素含量.
Synthesis of fluorescent carbon dots by electrochemical method for chloramphenicol probe
Using glucose as carbon source,the C-dots were synthesized by electrochemical carbonization method and the morphology and optical properties of C-dots were characterized.The prepared C-dots were used as fluorescence probe to detect the chloramphenicol.The results show that the as-prepared C-dots emit blue fluorescence when excited by ultraviolet light(excitation/emission wavelength was 345/450 nm,respectively).The chloramphenicol has quenching effect on the fluorescence intensity of C-dots and the quenching effect of chloramphenicol on the fluorescence intensity of C-dots vs the concentration display a good linear relationship,with a detection linear range of 0.1~5.0 mmol/L and a lower limit of detection of 0.016 mmol/L.The as-prepared C-Dots have excellent fluorescence property and optical stability,which can be used for the detection of chloramphenicol content sensitively and specifically.

electrochemical methodcarbon dotschloramphenicol detectionfluorescence probepharmaceutical analysis

何芳、张颖、张运良、孙双姣

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邵阳学院 药学院,湖南 邵阳,422000

湘西南中药开发利用湖南省工程研究中心,湖南 邵阳,422000

电化学法 碳点 氯霉素检测 荧光探针 药物分析

湖南省教育厅优秀青年项目湖南省区域联合基金湖南省区域联合基金湖南省区域联合基金

21B06752022JJ502032023JJ502532022JJ50233

2024

邵阳学院学报(自然科学版)
邵阳学院

邵阳学院学报(自然科学版)

影响因子:0.286
ISSN:1672-7010
年,卷(期):2024.21(1)
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