Clinical significance of miR-28-3p expression in hepatocellular carcinoma tissues and its impact on proliferation,invasion and migration of hepatocellular carcinoma cells
Objective To study the relationship between miR-28-3p expression in hepatocellular carcinoma(HCC)and HCC patients'clinicopathological characteristics and prognosis,and then to explore the effect of miR-28-3p expression on the proliferation,invasion,and migration of HCC cells.Methods MiR-28-3p expression data of HCC,clinicopathological parameters,and survival information were downloaded from the Cancer Genome Atlas(TCGA)database,and the relationships between miR-28-3p expression and clinicopathological parameters and prognosis were analyzed respectively.Chemically synthesized miR-28-3p mimics and negative control were used to transfect HCC cells line Huh7,and then cell counting Kit-8(CCK-8)and tranwell invasion and migration assays were employed to detect the proliferation,invasion and migration abilities of the cells in each group.Results MiR-28-3p was highly expressed in HCC tissues with a liver fibrosis background,and its expression level was significantly correlated with tumor size and TNM stage(P<0.05).Patients with high expression of miR-28-3p had a worse prognosis(P<0.05).As Cox analysis shows,miR-28-3p is an independent prognostic factor for patients with HCC.Vitro cell experiments found that,compared with the mimics NC,transfection of miR-28-3p mimics can significantly improved the proliferation,invasion,and migration abilities of HCC cells(all P<0.05).Conclusion MiR-28-3p is an independent predictor of poor prognosis of HCC,and high expression of miR-28-3p promotes the proliferation,invasion,and migration capabilities of HCC cells.
miR-28-3phepatocellular carcinoma(HCC)prognosisproliferationinvasion and migration
万方数据
维普
