Risk factors of spontaneous bacterial peritonitis and peripheral blood mononuclear cell CD36/mTORC1 signal pathway expression in patients with decompensated cirrhosis
Objective The purpose of this study was to investigate the risk factors of spontaneous bacterial peritonitis(SBP)and peripheral blood mononuclear cell(PBMC)cluster of differentiation(CD)36/mammalian target of rapamycin 1(mTORC1)signal pathway expression in patients with decompensated cirrhosis.Methods 82 patients with decompensated liver cirrhosis were encountered in our hospital between July 2020 and December 2023.Ascites bacteria was cultured and characterized routinely.The PBMCs were separated and the CD36/mTORC1 mRNA was detected by real-time fluorescent quantitative PCR.The multivariate Logistic regression analysis was applied to screen the risk factors for SBP occurrence in patients with decompensated cirrhosis.Results The complications of SBP was found in 43 cases out of our patients with decompensated liver cirrhosis and eight strains(9.8%)of bacteria were successfully isolated,including one strain of Staphylococcus coriolis,one strain of Hemolytic Staphylococcus,two strains of Escherichia coli,two strains of Klebsiella pneumonia and two strains of Enterobacter cloacae;the incidence of past SBP,serum total bilirubin,albumin,international normalized ratio,the model of end-stage liver disease score,the PBMC CD36 mRNA and mTORC1 mRNA in patients with SBP were 51.2%,(45.7±5.2)μmol/L,(21.7±3.1)g/L,(1.5±0.5),(24.9±7.5),(3.2±0.8)and(2.4±0.7),all significantly different compared to[18.0%,(12.3±1.4)μmol/L,(35.3±5.4)g/L,(1.2±0.3),(12.8±3.7),(1.4±0.5)and(1.1±0.4),respectively,P<0.05]in patients with liver cirrhosis;the multivariate Logistic regression analysis showed that the past SBP,serum bilirubin,albumin,MELD score as well as PBMC CD36 and mTORC1 levels were all the independent risk factors for the occurrence of SBP in patients with decompensated liver cirrhosis(P<0.05).Conclusion The PBMC CD36/mTORC1 signal pathway is up-regulated in patients with decompensated cirrhosis and complicated SBP,and the mechanism involved in the pathogenesis needs further investigation.
Decompensated liver cirrhosisSpontaneous bacterial peritonitisCluster of differentiation 36/mammalian target of rapamycin 1 signal pathway
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